Human umbilical cord blood cells directly suppress ischemic oligodendrocyte cell death.
Human umbilical cord blood cells directly suppress ischemic oligodendrocyte cell death.
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DOI：
10.1002/jnr.21857
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发表时间：
2009-02
影响因子：
4.2
通讯作者：
Pennypacker, K. R.
中科院分区：
文献类型：
--
作者：
Hall, A. A.;Guyer, A. G.;Leonardo, C. C.;Ajmo, C. T., Jr.;Collier, L. A.;Willing, A. E.;Pennypacker, K. R.
以往的报道表明，在大脑中动脉闭塞后48小时静脉注射人脐血细胞(HUCBCs)可减少啮齿动物的脑梗塞面积和行为缺陷。这种细胞疗法具有强大的神经保护和抗炎作用。本研究观察HUCBC对脑缺血大鼠脑白质损伤和少突胶质细胞存活的影响。从组织切片髓鞘碱性蛋白染色的定量可以看出，卒中后48小时静脉输注106 HUCBCs可减少体内白质损伤量。为了确定HUCBC是否通过直接作用于少突胶质细胞而起到保护作用，我们在体外缺氧缺糖模型中对培养的少突胶质细胞进行了研究。采用活性caspase3免疫组织化学方法和乳酸脱氢酶细胞毒实验检测HUCBCs对体外培养的少突胶质细胞的保护作用。根据这些结果，HUCBC给药可能直接保护少突胶质细胞和白质。这种效应可能有助于HUCBC治疗所观察到的行为恢复的增加。
Previous reports have shown that human umbilical cord blood cells (HUCBCs) administered intravenously 48 hrs following middle cerebral artery occlusion reduces infarct area and behavioral deficits of rodents. This cellular therapy is potently neuroprotective and anti-inflammatory. This study investigates the effect of HUCBC treatment on white matter injury and oligodendrocyte survival in a rat model of ischemia. Intravenous infusion of 106 HUCBCs 48 hrs post-stroke reduced the amount of white matter damage in vivo as seen by quantification of myelin basic protein staining in tissue sections. To determine whether HUCBC treatment was protective via direct effects on oligodendrocytes, cultured oligodendrocytes were studied in an in vitro model of oxygen glucose deprivation. Active caspase 3 immunohistochemistry and the LDH assay for cytotoxicity were used to determine that HUCBCs provide protection to oligodendrocytes in vitro. Based on these results, it is likely that HUCBC administration directly protects oligodendrocytes and white matter. This effect is likely to contribute to the increased behavioral recovery observed with HUCBC therapy.
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影响因子：
2.1
作者：
Ajmo, Craig T., Jr.;Vernon, Dionne O. L.;Cuevas, Javier
通讯作者：
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DOI：
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发表时间：
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影响因子：
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发表时间：
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