A resting pool of vesicles is responsible for spontaneous vesicle fusion at the synapse.
A resting pool of vesicles is responsible for spontaneous vesicle fusion at the synapse.
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DOI：
10.1038/nn.2317
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发表时间：
2009-06
影响因子：
25
通讯作者：
Burrone, Juan
中科院分区：
文献类型：
--
作者：
Fredj, Naila Ben;Burrone, Juan
突触通过释放储存在突触前囊泡中的神经递质传递信息。已经使用各种技术研究了由神经元活动动员的囊泡池的身份、动力学和位置。在这里，我们描述了一种新的基因编码的探针，biosyn，它由生物素化的VAMP-2在突触前末端表达。我们利用链霉亲和素和生物素之间的高亲和力相互作用，在囊泡融合过程中用荧光链霉亲和素标记生物素。这种方法允许标记囊泡顺序，可视化和建立突触前池的身份。使用这种技术，我们能够区分两个不同的池的囊泡在大鼠海马神经元：一个是释放响应突触前活动和另一个，不同的囊泡池，自发地与质膜融合。我们进一步确定，自发囊泡属于一个“休息池”，通常不动员神经元的活动，其功能大多是未知的。
Synapses relay information through the release of neurotransmitters stored in presynaptic vesicles. The identity, kinetics and location of vesicle pools mobilized by neuronal activity have been studied using a variety of techniques. Here, we describe a novel genetically-encoded probe, biosyn, which consists of a biotinylated VAMP-2 expressed at presynaptic terminals. We exploit the high affinity interaction between streptavidin and biotin to label biosyn with fluorescent streptavidin during vesicle fusion. This approach allows tagging of vesicles sequentially, to visualize and establish the identity of presynaptic pools. Using this technique we were able to distinguish between two different pools of vesicles in rat hippocampal neurons: one that is released in response to presynaptic activity and another, distinct vesicle pool that spontaneously fuses with the plasma membrane. We further established that the spontaneous vesicles belong to a ‘resting pool’ that is normally not mobilized by neuronal activity and whose function is mostly unknown.
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影响因子：
3.5
作者：
Predonzani A;Arnoldi F;López-Requena A;Burrone OR
通讯作者：
Burrone OR
DOI：
10.1007/s11068-008-9030-y
发表时间：
2008-08-01
期刊：
BRAIN CELL BIOLOGY
影响因子：
--
作者：
Fernandez-Alfonso, Tomas;Ryan, Timothy A.
通讯作者：
Ryan, Timothy A.
影响因子：
4.7
作者：
Poskanzer, KE;Davis, GW
通讯作者：
Davis, GW
影响因子：
64.5
作者：
GEPPERT, M;GODA, Y;SUDHOF, TC
通讯作者：
SUDHOF, TC
影响因子：
48
作者：
Chen, I;Howarth, M;Ting, AY
通讯作者：
Ting, AY