Effect of white spot syndrome virus infection on a Ras gene in the Chinese shrimp Fenneropenaeus chinensis
Effect of white spot syndrome virus infection on a Ras gene in the Chinese shrimp Fenneropenaeus chinensis
复制标题
白斑综合症病毒感染对中国明对虾Ras基因的影响
DOI：
10.1016/j.aquaculture.2019.734604
复制
发表时间：
2020-02
期刊：
影响因子：
4.5
通讯作者：
Kong Jie
中科院分区：
文献类型：
--
作者：
Li Xupeng;Meng Xianhong;Luan Sheng;Luo Kun;Cao Baoxiang;Chen Baolong;Kong Jie
Ras是一种小的GTP结合蛋白，其参与多种细胞过程的调节，包括细胞周期进展、增殖和凋亡。本研究克隆了中国明对虾（Fenneropenaeus chinensis）Ras蛋白的cDNA（命名为FcRas）。FcRa基因cDNA包含一个564 bp的开放阅读框（ORF）、5个GTP代谢相关保守区和一个CAAX基序。多重序列比对显示Ras的C端保守性低于N端。健康中国对虾肝胰腺和鳃中FcRasmRNA的表达量显著高于肌肉中的表达量（P< 0.05）。用白色斑点综合征病毒（WSSV）攻击后，肌肉中FcRasmRNA水平在感染后48 h显著上调（P< 0.05）。肝胰腺FcRasmRNA表达在感染后12 h（P< 0.01）和24 h（P< 0.05）显著下调。肝胰腺FcRas蛋白表达在感染后6、12、24和48 h显著下调（P< 0.05）。这些结果表明，WSSV感染对FcRas表达水平有影响。
Ras is a small GTP-binding protein that is involved in the regulation of diverse cellular processes, including cell cycle progression, proliferation, and apoptosis. In the present study, a cDNA encoding Ras protein in Chinese shrimp Fenneropenaeus chinensis (designatedFcRas) was cloned. The cDNA ofFcRascontained a 564 bp open reading frame (ORF), five conserved regions involved in GTP metabolism, and a CAAX motif. Multiple sequence alignment showed that the C-terminal of Ras was less conserved than the N-terminal. In healthyF.chinensis, theFcRasmRNA level in the hepatopancreas and gill were both significantly (P< 0.05) higher than the level in muscle. Following challenge with white spot syndrome virus (WSSV), theFcRasmRNA level in the muscle was significantly (P< 0.05) upregulated at 48 h post-infection (hpi). In the hepatopancreas, theFcRasmRNA level was significantly downregulated at 12 hpi (P< 0.01) and 24 hpi (P< 0.05). The protein level of FcRas in the hepatopancreas was significantly downregulated at 6, 12, 24, and 48 hpi (P< 0.05). These results suggested that theFcRasexpression level was influenced by WSSV infection.
登录
查看更多内容
影响因子：
64.5
作者：
Heidorn SJ;Milagre C;Whittaker S;Nourry A;Niculescu-Duvas I;Dhomen N;Hussain J;Reis-Filho JS;Springer CJ;Pritchard C;Marais R
通讯作者：
Marais R
影响因子：
5.2
作者：
F. Chang;L. Steelman;J. G. Shelton;John T. Lee;P. Navolanic;W. Blalock;R. Franklin;J. McCubrey
通讯作者：
F. Chang;L. Steelman;J. G. Shelton;John T. Lee;P. Navolanic;W. Blalock;R. Franklin;J. McCubrey
影响因子：
4.7
作者：
Leilei Wang;Ling Li;Lingling Wang;Jialong Yang;Jingjing Wang;Zhi Zhou;Huan Zhang;L. Song
通讯作者：
Leilei Wang;Ling Li;Lingling Wang;Jialong Yang;Jingjing Wang;Zhi Zhou;Huan Zhang;L. Song
影响因子：
2.9
作者：
Shin-jen Lin;Hui-Lan Hsia;Wang-Jing Liu;Jiun-Yan Huang;Kuan‐Fu Liu;Wei-Yu Chen;Ying-Chun Yeh;Yun‐Tzu Huang;C. Lo;G. Kou;H. Wang
通讯作者：
Shin-jen Lin;Hui-Lan Hsia;Wang-Jing Liu;Jiun-Yan Huang;Kuan‐Fu Liu;Wei-Yu Chen;Ying-Chun Yeh;Yun‐Tzu Huang;C. Lo;G. Kou;H. Wang
影响因子：
11.2
作者：
E. Rozenblum;M. Schutte;M. Goggins;S. Hahn;Shawn Panzer;M. Zahurak;S. Goodman;T. Sohn;R. Hruban;C. Yeo;S. Kern
通讯作者：
E. Rozenblum;M. Schutte;M. Goggins;S. Hahn;Shawn Panzer;M. Zahurak;S. Goodman;T. Sohn;R. Hruban;C. Yeo;S. Kern