Impact of TiO2 nanoparticles on growth, biofilm formation, and flavin secretion in Shewanella oneidensis.
Impact of TiO2 nanoparticles on growth, biofilm formation, and flavin secretion in Shewanella oneidensis.
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DOI：
10.1021/ac400486u
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发表时间：
2013-06-18
影响因子：
7.4
通讯作者：
Haynes, Christy L.
中科院分区：
文献类型：
--
作者：
Maurer-Jones, Melissa A.;Gunsolus, Ian L.;Meyer, Ben M.;Christenson, Cole J.;Haynes, Christy L.
了解纳米颗粒对关键细菌功能的影响，使我们能够获得对毒性的机械理解，并指导我们制定创建安全纳米材料的设计规则。在此，并使用分析技术，生物膜形成，一般细菌功能，和核黄素分泌，物种特异性功能，监测希瓦氏菌oneidensis，金属还原细菌，暴露于各种TiO 2纳米颗粒类型（合成，Aeroxide P25，和T-Eco）。TEM图像显示，给药的纳米颗粒非常接近细菌，但它们没有内化。利用石英晶体微天平（QCM）研究表明，S。在纳米颗粒的存在下，延缓了oneidensis生物膜的形成。虽然S. oneidensis在TiO 2纳米颗粒存在下生长更慢，核黄素分泌，与S.纳米颗粒的金属还原能力以纳米颗粒剂量依赖性方式显著增加。生物膜形成和核黄素分泌的变化都得到了暴露于纳米颗粒的S. oneidensis。随着工程纳米颗粒使用的增加，这项对细菌纳米毒性的广泛研究，包括使用灵敏的分析工具对生物膜形成、核黄素分泌和基因表达进行功能评估，对整个生态系统的健康产生了影响。
Understanding of nanoparticle impacts on critical bacteria functions allows us to gain a mechanistic understanding of toxicity and guides us towards design rules for creating safe nanomaterials. Herein and using analytical techniques, biofilm formation, a general bacteria function, and riboflavin secretion, a species-specific function, were monitored in Shewanella oneidensis, a metal reducing bacterium, following exposure to a variety of TiO2 nanoparticle types (synthesized, Aeroxide P25, and T-Eco). TEM images show that dosed nanoparticles are in close proximity to the bacteria but they are not internalized. Using quartz crystal microbalance (QCM), it was revealed that S. oneidensis biofilm formation is slowed in the presence of nanoparticles. Though S. oneidensis grows more slowly in the presence of TiO2 nanoparticles, riboflavin secretion, a function related to the S. oneidensis metal reducing capacity, was increased significantly in a nanoparticle dose-dependent manner. Both changes in biofilm formation and riboflavin secretion are supported by changes in gene expression in nanoparticle-exposed S. oneidensis. This broad study of bacterial nanotoxicity, including use of sensitive analytical tools for functional assessments of biofilm formation, riboflavin secretion, and gene expression has implications for total ecosystem health as the use of engineered nanoparticles grows.
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影响因子：
4.4
作者：
Pelletier, Dale A.;Suresh, Anil K.;Doktycz, Mitchel J.
通讯作者：
Doktycz, Mitchel J.
影响因子：
5.1
作者：
McLean, J. S.;Pinchuk, G. E.;Beliaev, A. S.
通讯作者：
Beliaev, A. S.
影响因子：
1.8
作者：
Gong, Amy S.;Bolster, Carl H.;Walker, Sharon L.
通讯作者：
Walker, Sharon L.
影响因子：
3.2
作者：
Myers, CR;Myers, JM
通讯作者：
Myers, JM
影响因子：
--
作者：
Jiang, Guoxiang;Shen, Zhenyao;He, Tiande
通讯作者：
He, Tiande