Noninvasive MRI-SERS imaging in living mice using an innately bimodal nanomaterial.
Noninvasive MRI-SERS imaging in living mice using an innately bimodal nanomaterial.
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DOI：
10.1021/nn102587h
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发表时间：
2011-02-22
期刊：
影响因子：
17.1
通讯作者：
Medarova Z
中科院分区：
文献类型：
--
作者：
Yigit MV;Zhu L;Ifediba MA;Zhang Y;Carr K;Moore A;Medarova Z
我们报道了一种新型的纳米材料(AuMN-DTTC)，它可以用作体内磁共振成像(MRI)和拉曼光谱的双峰造影剂。该探针由具有核磁共振活性的超顺磁性氧化铁纳米颗粒组成，稳定地与金纳米结构络合。该金组分用作拉曼活性染料分子的底物以产生表面增强拉曼散射(SERS)效应。合成的探针具有T2加权对比度，在硅胶(水溶液)和体内均可用作SERS活性物质。利用多回声磁共振成像技术对T2弛豫时间进行定量评估。AUMN-DTTC和MN(葡聚糖包覆纳米氧化铁)的T2弛豫时间分别为29.23±1.45和31.58±1.7毫秒。AUMN-DTTC在508、629、782、844、1080、1108、1135和1242cM−1处出现峰值。肌肉注射该探针后，肌肉的T2松弛时间从33.4±2.5ms缩短到20.3±2.2ms。在508、629、782、844、1080、1108、1135和1242 cm−-1处观察到了表面增强拉曼散射峰，这与电子显微镜的结果一致。我们的研究首次展示了造影剂的设计和体内应用，其成分包括磁共振成像和表面增强拉曼散射。这种试剂的价值在于它天生的双峰性，以及它在体内应用于分子成像的应用。
We report a novel nanomaterial (AuMN-DTTC) that can be used as a bimodal contrast agent for in vivo magnetic resonance imaging (MRI) and Raman spectroscopy. The probe consists of MRI-active superparamagnetic iron oxide nanoparticles, stably complexed with gold nanostructures. The gold component serves as a substrate for a Raman active dye molecule to generate a surface-enhanced Raman scattering (SERS) effect. The synthesized probe produces T2 weighted contrast and can be used as a SERS active material both in silico (in aqueous solution) and in vivo. A quantitative assessment of T2 relaxation times was obtained using multiecho MRI analysis. The T2 relaxation times of AuMN-DTTC and MN (dextran-coated iron oxide nanoparticles) were 29.23 + 1.45, and 31.58 + 1.7 msec, respectively. The SERS signature of AuMN-DTTC revealed peaks at 508, 629, 782, 844, 1080, 1108, 1135, and 1242 cm−1. Intramuscular administration of the probe resulted in a decrease of the T2 relaxation time of muscle from 33.4 + 2.5 msec to 20.3 + 2.2. SERS peaks were observed at 508, 629, 782, 844, 1080, 1108, 1135, and 1242 cm−1, consistent with the in silico results. Our studies illustrate for the first time the design and in vivo application of a contrast agent, whose component modalities include MRI and SERS. The value of this agent lies in its innately bimodal nature and its application in vivo for molecular imaging applications.
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影响因子：
11.2
作者：
Medarova Z;Rashkovetsky L;Pantazopoulos P;Moore A
通讯作者：
Moore A
影响因子：
64.8
作者：
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Storhoff, JJ
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作者：
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影响因子：
158.5
作者：
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通讯作者：
Weissleder, R
影响因子：
15
作者：
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通讯作者：
Brus, LE