Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis.
Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis.
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DOI：
10.1016/j.joca.2013.06.031
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发表时间：
2013-09
影响因子：
7
通讯作者：
Walsh, D. A.
中科院分区：
文献类型：
--
作者：
Mapp, P. I.;Sagar, D. R.;Ashraf, S.;Burston, J. J.;Suri, S.;Chapman, V.;Walsh, D. A.
描述两种骨关节炎（OA）大鼠模型之间关节病理学和疼痛行为的差异，以便为干预研究选择动物模型提供信息。在Sprague道利大鼠中，通过膝关节横断（MNX）或关节内注射碘乙酸钠（MIA）诱导膝关节OA。对照组进行假手术或盐水注射。在另一项实验中，在MNX或MIA关节炎诱导后14天，给予单次关节内注射曲安奈德。量化疼痛行为和关节病理学。两种模型均显示滑膜炎症、软骨病和骨赘病。在两种模型中，软骨病评分随时间增加相似。与MIA模型相比，MNX模型中的炎症和骨赘评分更高。在第49天，与所有其他组相比，MNX模型表现出更多数量的通道穿过骨软骨连接。与MIA模型相比，MNX模型表现出更大的承重不对称性，而MIA模型表现出更一致的后爪异常性疼痛。在MNX模型中，曲安西龙将负重不对称性和远端异常性疼痛减弱至对照水平，但在MIA模型中远端异常性疼痛没有改变。使用相同的评估工具对大鼠中的两种OA模型进行的比较表明，尽管两种模型均显示出OA的特征，但模型之间存在差异，可能代表人类OA的不同方面。因此，模型的选择应基于所研究的OA的病理学方面。
To characterize differences in joint pathology and pain behavior between two rat models of osteoarthritis (OA) in order to inform selection of animal models for interventional studies. Knee OA was induced in Sprague Dawley rats by either meniscal transection (MNX) or intra-articular injection of monosodium iodoacetate (MIA). Controls were subjected to sham surgery or saline-injection. In a separate experiment, a single intra-articular injection of triamcinolone acetonide was administered 14 days after MNX or MIA arthritis induction. Pain behavior and joint pathology were quantified. Both models displayed synovial inflammation, chondropathy and osteophytosis. Chondropathy scores increased with time similarly in the two models. Inflammation and osteophyte scores were greater in MNX model compared to the MIA model. At day 49, the MNX model exhibited a greater number of channels crossing the osteochondral junction compared to all other groups. The MNX model exhibited greater weight bearing asymmetry compared to the MIA model, whereas the MIA model displayed more consistent hindpaw allodynia. Triamcinolone attenuated weight bearing asymmetry and distal allodynia to control levels in the MNX model, but distal allodynia was unaltered in the MIA model. The comparison of the two models of OA in rats, using identical assessment tools has demonstrated that although both models display features of OA, there are differences between the models which may represent different aspects of human OA. Thus, model selection should be based on the pathological aspects of OA under investigation.
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影响因子：
7.4
作者：
Ivanavicius, Stefan P.;Ball, Adrian D.;Read, Simon J.
通讯作者：
Read, Simon J.
影响因子：
--
作者：
Hwang, Jennifer;Bae, Won C.;Shieu, Wendy;Lewis, Chad W.;Bugbee, William D.;Sah, Robert L.
通讯作者：
Sah, Robert L.
影响因子：
--
作者：
Guingamp, C;GegoutPottie, P;Gillet, P
通讯作者：
Gillet, P
影响因子：
2.7
作者：
Rossi, Heather L.;Neubert, John K.
通讯作者：
Neubert, John K.
影响因子：
7.4
作者：
SELTZER, Z;DUBNER, R;SHIR, Y
通讯作者：
SHIR, Y