Increased hydraulic conductance of human articular cartilage and subchondral bone plate with progression of osteoarthritis.
Increased hydraulic conductance of human articular cartilage and subchondral bone plate with progression of osteoarthritis.
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DOI：
10.1002/art.24069
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发表时间：
2008-12
影响因子：
--
通讯作者：
Sah, Robert L.
中科院分区：
文献类型：
--
作者：
Hwang, Jennifer;Bae, Won C.;Shieu, Wendy;Lewis, Chad W.;Bugbee, William D.;Sah, Robert L.
骨关节炎（OA）的特征在于关节软骨的进行性退变和软骨下骨板（ScBP）的重塑，所述软骨下骨板（ScBP）由钙化软骨（CC）和下面的软骨下骨（ScB）组成。CC重塑由于向上侵犯血管管或CC侵蚀可能有助于骨软骨（OC）组织及其ScBP成分的生物力学改变。研究假设是OC组织和ScBP的水力传导性随着OA阶段增加的结构变化而增加。从尸体组织供体的膝关节和OA膝关节手术患者的废弃碎片中获取OC核心。组织供体芯肉眼观察正常，OA芯对骨有部分或全层侵蚀。对核心进行灌注测试，以确定在其天然状态下和酶促去除软骨后的水力传导性或流体流动的容易程度。通过3-D组织学分析相邻部分的CC、ScB和ScBP厚度和血管管密度。完全侵蚀样本中天然OC组织和ScBP的水力传导比正常样本高（2700倍和3倍）。CC层厚（1.5倍）在部分侵蚀的样品比正常的样品，但在完全侵蚀的样品薄，不完整。ScBP血管分布随着OA分期的增加而改变。在关节负荷期间，OC组织和ScBP的水力传导性增加可能对软骨产生有害的生物力学后果。来自覆盖和相对软骨的液体渗出增加、液体减压增加和软骨组织应变增加可导致软骨细胞死亡和软骨损伤。
Osteoarthritis (OA) is characterized by progressive degeneration of articular cartilage and remodeling of the subchondral bone plate (ScBP), comprised of calcified cartilage (CC) and underlying subchondral bone (ScB). CC remodeling due to upward invasion by vascular canals or to CC erosion may contribute to biomechanical alteration of the osteochondral (OC) tissue and its ScBP component. The study hypothesis was that hydraulic conductance of OC tissue and ScBP increases with structural changes indicative of increasing stages of OA. OC cores were harvested from knees of cadaveric tissue donors and from discarded fragments of OA knee surgery patients. Tissue donor cores were macroscopically normal, and OA cores had partial- or full-thickness erosion to bone. Cores were perfusion-tested to determine the hydraulic conductance, or ease of fluid flow, in their native state and after enzymatic removal of cartilage. Adjacent portions were analyzed by 3-D histology for CC, ScB, and ScBP thickness and vascular canal density. The hydraulic conductances of native OC tissue and ScBP were higher (2700- and 3-fold) in fully eroded samples than normal samples. The CC layer was thicker (1.5-fold) in partially eroded samples than normal samples, but thinner and incomplete in fully eroded samples. ScBP vascularity was altered with increasing stages of OA. During joint loading, increased hydraulic conductance of the OC tissue and ScBP could have deleterious biomechanical consequences for cartilage. Increased fluid exudation from overlying and opposing cartilage, increased fluid depressurization, and increased cartilage tissue strains could lead to chondrocyte death and cartilage damage.
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影响因子：
2.8
作者：
Ayotte, DC;Ito, K;Tepic, S
通讯作者：
Tepic, S
影响因子：
3.2
作者：
Jadin, KD;Wong, BL;Sah, RL
通讯作者：
Sah, RL
DOI：
10.1302/0301-620x.35b4.643
发表时间：
1953-01-01
影响因子：
--
作者：
LANDELLS, JW
通讯作者：
LANDELLS, JW
影响因子：
39.2
作者：
Felson, DT;Chaisson, CE;Gale, DR
通讯作者：
Gale, DR
影响因子：
2.8
作者：
Keinan-Adamsky, K;Shinar, H;Navon, G
通讯作者：
Navon, G