Moderate hypothermia inhibits brain inflammation and attenuates stroke-induced immunodepression in rats.
Moderate hypothermia inhibits brain inflammation and attenuates stroke-induced immunodepression in rats.
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DOI：
10.1111/cns.12160
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发表时间：
2014-01
影响因子：
5.5
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Gu LJ;Xiong XX;Ito T;Lee J;Xu BH;Krams S;Steinberg GK;Zhao H
中风会导致脑部炎症和免疫抑制。众所周知，亚低温可以减轻脑部炎症，但它在中风诱导的外周免疫系统免疫抑制(SID)中的作用尚不清楚。本研究观察了大鼠脑缺血后亚低温对SID及脑组织炎症的影响。采用大脑中动脉远端永久性结扎结合短暂双侧颈总动脉结扎的方法，在体温降至30°C的条件下建立大鼠卒中模型。采用实时荧光定量聚合酶链式反应、流式细胞仪、体外T细胞增殖实验和共聚焦显微镜观察SID和脑组织炎症反应。短暂的缺血内低温有助于维持外周血和脾中的某些白细胞，促进T细胞体外增殖和体内迟发型超敏反应，提示低温可降低SID。相反，在脑内，短暂的缺血低温抑制了抗炎细胞因子IL-10和促炎细胞因子INF-γ、肿瘤坏死因子-α、IL-2、IL-1β和MIP-2的基因表达。短暂的缺血内低温还可减轻淋巴细胞、中性粒细胞(MPO+细胞)和巨噬细胞(CD68+细胞)在缺血脑内的渗透，提示低温可抑制脑组织炎症反应。短暂的缺血内低温可减轻SID并保护其免受急性脑炎的侵袭。
Stroke causes both brain inflammation and immunodepression. Mild to moderate hypothermia is known to attenuate brain inflammation but its role in stroke-induced immunodepression (SIID) of the peripheral immune system remains unknown. This study investigated the effects in rats of moderate intra-ischemic hypothermia on SIID and brain inflammation. Stroke was induced in rats by permanent distal MCA occlusion combined with transient bilateral CCA occlusion while body temperature was reduced to 30°C. Real-time PCR, flow cytometry, in vitro T cell proliferation assays and confocal microscopy were used to study SIID and brain inflammation. Brief Intra-Ischemic hypothermia helped maintain certain leukocytes in the peripheral blood and spleen, and enhanced T cell proliferation in vitro and delayed-type hypersensitivity in vivo, suggesting that hypothermia reduces SIID. In contrast, in the brain, brief intra-Ischemic hypothermia inhibited mRNA expression of anti-inflammatory cytokine IL-10 and pro-inflammatory cytokines INF-γ, TNF-α, IL-2, IL-1β and MIP-2. Brief intra-Ischemic hypothermia also attenuated the infiltration of lymphocytes, neutrophils (MPO+ cells) and macrophages (CD68+ cells) into the ischemic brain, suggesting that hypothermia inhibited brain inflammation. Brief intra-ischemic hypothermia attenuated SIID and protected against acute brain inflammation.
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影响因子：
8.3
作者：
Maier, CM;Ahern, KV;Steinberg, GK
通讯作者：
Steinberg, GK
影响因子：
8.3
作者：
Gelderblom, Mathias;Leypoldt, Frank;Magnus, Tim
通讯作者：
Magnus, Tim
影响因子：
6.3
作者：
Weston, Robert M.;Jones, Nicole M.;Callaway, Jennifer K.
通讯作者：
Callaway, Jennifer K.
DOI：
10.1084/jem.20021098
发表时间：
2003-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Prass K;Meisel C;Höflich C;Braun J;Halle E;Wolf T;Ruscher K;Victorov IV;Priller J;Dirnagl U;Volk HD;Meisel A
通讯作者：
Meisel A
影响因子：
4.4
作者：
Offner, Halina;Subramanian, Sandhya;Hurn, Patricia D.
通讯作者：
Hurn, Patricia D.