Antigen Discovery for Next-Generation Pertussis Vaccines Using Immunoproteomics and Transposon-Directed Insertion Sequencing.
Antigen Discovery for Next-Generation Pertussis Vaccines Using Immunoproteomics and Transposon-Directed Insertion Sequencing.
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使用免疫蛋白质组学和转座子定向插入测序发现下一代百日咳疫苗的抗原。
DOI：
10.1093/infdis/jiac502
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sette,Alessan
中科院分区：
文献类型：
--
作者：
Gregg,KelseyA;Wang,Yihui;Warfel,Jason;Schoenfeld,Elizabeth;Jankowska,Ewa;Cipollo,JohnF;Mayho,Matthew;Boinett,Christine;Prasad,Deepika;Brickman,TimothyJ;Armstrong,SandraK;Parkhill,Julian;DaSilvaAntunes,Ricardo;Sette,Alessan
背景尽管疫苗接种率很高，但在改用无细胞百日咳疫苗后，美国报告的百日咳病例再次出现，表明需要改进疫苗以加强感染控制。方法通过免疫蛋白质组学鉴定恢复期狒狒血清和鼻咽洗液识别的百日咳博德特氏菌抗原并预测其亚细胞定位。通过转座子定向插入位点测序 (TraDIS) 分析鉴定了对狒狒气道持久存在必需或重要的基因。结果总计 314B。第748章 百日咳抗原TraDIS 鉴定出 13 种狒狒具有免疫原性，对于在气道中持续存在至关重要，并且是膜定位的：BP0840 (OmpP)、Pal、OmpA2、BP1485、BamA、Pcp、MlaA、YfgL、BP2197、BP1569、MlaD、ComL 和 BP0183。百日咳抗原被确定为免疫原性、在气道中持续存在所必需的、并且是膜定位的，值得进一步研究，以将其纳入旨在减少或防止接种个体气道中细菌携带的疫苗中。
BackgroundDespite high vaccination rates, the United States has experienced a resurgence in reported cases of pertussis after switching to the acellular pertussis vaccine, indicating a need for improved vaccines that enhance infection control.MethodsBordetella pertussisantigens recognized by convalescent-baboon serum and nasopharyngeal wash were identified by immunoproteomics and their subcellular localization predicted. Genes essential or important for persistence in the baboon airway were identified by transposon-directed insertion-site sequencing (TraDIS) analysis.ResultsIn total, 314B. pertussisantigens were identified by convalescent baboon serum and 748 by nasopharyngeal wash. Thirteen antigens were identified as immunogenic in baboons, essential for persistence in the airway by TraDIS, and membrane-localized: BP0840 (OmpP), Pal, OmpA2, BP1485, BamA, Pcp, MlaA, YfgL, BP2197, BP1569, MlaD, ComL, and BP0183.ConclusionsTheB. pertussisantigens identified as immunogenic, essential for persistence in the airway, and membrane-localized warrant further investigation for inclusion in vaccines designed to reduce or prevent carriage of bacteria in the airway of vaccinated individuals.
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作者：
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作者：
ELLIS, EN;STEFFES, MW;MAUER, SM
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影响因子：
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PARVING, HH;ANDERSEN, AR;SVENDSEN, PA
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SVENDSEN, PA
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作者：
Charles L. Kunzelman;W. Knowler;D. Pettitt;P. Bennett
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DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
E. Ritz;C. Hasslacher;JohannesF. E. Mann
通讯作者：
JohannesF. E. Mann