Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation [published erratum appears in J Cell Biol 1998 Dec 28;143(7):following 2090]
Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation [published erratum appears in J Cell Biol 1998 Dec 28;143(7):following 2090]
复制标题
小脑颗粒细胞中三碘甲腺原氨酸诱导的 Neurotropin-3 促进浦肯野细胞分化 [已发表的勘误表出现在 J Cell Biol 1998 年 12 月 28 日；143(7)：2090 年之后]
作者：
D. Lindholm;Eero Castr~n;P. Tsoulfas;R. Kolbeck;M. da;Penha Berzaghi;Axel Leing 'drtner;C. Heisenberg;L. Tesarollo;L. Parada;H. Thoenen
甲状腺激素在脑发育中起重要作用，但三碘甲腺原氨酸(T3)介导神经元分化的机制(S)知之甚少。在这里，我们证明了在细胞培养和体内发育的大鼠小脑颗粒细胞中，T3调节神经营养因子神经营养因子-3(NT-3)。原位杂交实验表明，发育中的浦肯野细胞不表达NT-3mRNA，但表达trkC，这可能是NT-3的神经元受体。将重组NT-3加入胎鼠小脑培养物中，可诱导浦肯野细胞肥大和突起萌发，并上调钙结合蛋白Calbindin-28kD的表达。本研究证明了小脑颗粒神经元与发育中的浦肯野细胞之间的一种新的相互作用，即在颗粒细胞中由T3诱导的NT-3促进浦肯野细胞的分化。
Thyroid hormones play an important role in brain development, but the mechanism(s) by which triiodothyronine (T3) mediates neuronal differentiation is poorly understood. Here we demonstrate that T3 regulates the neurotrophic factor, neurotrophin-3 (NT-3), in developing rat cerebellar granule cells both in cell culture and in vivo. In situ hybridization experiments showed that developing Purkinje cells do not express NT-3 mRNA but do express trkC, the putative neuronal receptor for NT-3. Addition of recombinant NT-3 to cerebellar cultures from embryonic rat brain induces hypertrophy and neurite sprouting of Purkinje cells, and upregulates the mRNA encoding the calcium-binding protein, calbindin-28 kD. The present study demonstrates a novel interaction between cerebellar granule neurons and developing Purkinje cells in which NT-3 induced by T3 in the granule cells promotes Purkinje cell differentiation.
甲状腺激素受体蛋白β1和变体α2在选定组织中的免疫荧光定位：小脑浦肯野细胞作为大脑中甲状腺激素β1受体介导的发育效应的模型。
DOI：
10.1073/pnas.88.9.3887
发表时间：
1991
影响因子：
11.1
作者：
Strait,KA;Schwartz,HL;Seybold,VS;Ling,NC;Oppenheimer,JH
通讯作者：
Oppenheimer,JH
影响因子：
4.6
作者：
R. Klein;D. Martín-Zanca;M. Barbacid;L. Parada
通讯作者：
R. Klein;D. Martín-Zanca;M. Barbacid;L. Parada
甲状腺激素对基因表达的调节。
影响因子：
18.2
作者：
Samuels,HH;Forman,BM;Horowitz,ZD;Ye,ZS
通讯作者：
Ye,ZS