Selective autophagy and Golgi quality control in <i>Drosophila</i>
Selective autophagy and Golgi quality control in <i>Drosophila</i>
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DOI：
10.1080/15548627.2022.2098765
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发表时间：
2022-10
期刊：
影响因子：
13.3
通讯作者：
Nezis, Ioannis P.
中科院分区：
文献类型：
--
作者：
Gohel, Raksha;Rahman, Ashrafur;Lorincz, Peter;Nagy, Aniko;Csordas, Gabor;Zhang, Yan;Juhasz, Gabor;Nezis, Ioannis P.
at8 /LC3蛋白的LIR基序对接位点（LDS）对于LC3相互作用区（LIR）蛋白的结合及其随后的巨噬/自噬降解至关重要。在我们最近的研究中，我们在at8 /LC3的果蝇同系物Atg8a中创建了一个突变的LDS位点，发现LDS突变体积累了已知的自噬底物并缩短了寿命。我们还进行了定量蛋白质组学分析，并鉴定了几种在LDS突变体中富集的蛋白质，包括Gmap（高尔基微管相关蛋白）。Gmap包含一个LIR基序，并在LDS突变体中积累。我们发现Gmap和Atg8a以LIR-LDS依赖的方式相互作用，并且Atg8a- lds和Gmap- lir基序突变体的高尔基体大小和形态发生了改变。我们的发现强调了Gmap在贪食调节中的作用。
The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and found that LDS mutants accumulate known autophagy substrates and have reduced lifespan. We also conducted quantitative proteomics analyses and identified several proteins that are enriched in the LDS mutants, including Gmap (Golgi microtubule-associated protein). Gmap contains a LIR motif and accumulates in LDS mutants. We showed that Gmap and Atg8a interact in a LIR-LDS dependent manner and that the Golgi size and morphology are altered in Atg8a-LDS and Gmap-LIR motif mutants. Our findings highlight a role for Gmap in the regulation of Golgiphagy.
影响因子：
8.8
作者：
Rahman A;Lőrincz P;Gohel R;Nagy A;Csordás G;Zhang Y;Juhász G;Nezis IP
通讯作者：
Nezis IP