Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins.
Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins.
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DOI：
10.1186/1471-2164-8-421
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发表时间：
2007-11-15
期刊：
影响因子：
4.4
通讯作者：
Karrow NA
中科院分区：
文献类型：
--
作者：
Pant SD;Schenkel FS;Leyva-Baca I;Sharma BS;Karrow NA
Toll样受体-2(TLR2)和Caspase招募结构域15(CARD15)是重要的模式识别受体，在启动炎症反应和随后的免疫反应中发挥作用。它们以前被认为是人类炎症性肠病的易感基因，因此是牛抗炎性疾病的合适候选基因。本研究的目的是确定牛TLR2和CARD15的单核苷酸多态(SNPs)，并评估这些SNPs与加拿大荷斯坦公牛群体的健康和生产性状的关系。根据预估的SCS育种值(EBV)构建了一个选择性DNA池。在聚合酶链式反应中从该池中扩增出基因片段，并对扩增产物进行测序以揭示多态现象。在CARD15中共检测到4个SNP，其中1个位于内含子10(c.2886-14A>；G)，3个位于外显子12(c.3020A>；T、c.4500A>；C和c.4950C>；T)，在TLR2中未发现。对338头加拿大荷斯坦公牛进行了基因分型和单倍型重构。两个SNP c.3020A>；T和c.4500A>；C与EBV的健康和生产性状相关。例如，SNP c.3020A>；T与SCS EBV相关(p=0.0097)，等位基因替代效应为0.07Score。与最常见的单倍型Hap12(AC)相比，Hap22(TC)与产奶量(p<；0.0001)和蛋白质产量(p=0.0007)相关，Hap21(TA)与SCS EBV(p=0.0120)显著相关。通过排列检验，所有显著的比较关联在8%的实验水平上保持显著。本研究表明，SNP c.3020A>；T可能在宿主对乳房炎的反应中发挥作用，其作用机制还需要进一步详细的研究。
Toll-like receptor-2 (TLR2) and Caspase Recruitment Domain 15 (CARD15) are important pattern recognition receptors that play a role in the initiation of the inflammatory and subsequent immune response. They have been previously identified as susceptibility loci for inflammatory bowel diseases in humans and are, therefore, suitable candidate genes for inflammatory disease resistance in cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the bovine TLR2 and CARD15 and evaluate the association of these SNPs with health and production traits in a population of Canadian Holstein bulls. A selective DNA pool was constructed based on the estimated breeding values (EBVs) for SCS. Gene segments were amplified from this pool in PCR reactions and the amplicons sequenced to reveal polymorphisms. A total of four SNPs, including one in intron 10 (c.2886-14A>G) and three in the exon 12 (c.3020A>T, c.4500A>C and c.4950C>T) were identified in CARD15; none were identified in TLR2. Canadian Holstein bulls (n = 338) were genotyped and haplotypes were reconstructed. Two SNPs, c.3020A>T and c.4500A>C, were associated with EBVs for health and production traits. The SNP, c.3020A>T, for example, was associated with SCS EBVs (p = 0.0097) with an allele substitution effect of 0.07 score. When compared to the most frequent haplotype Hap12(AC), Hap22(TC) was associated with increased milk (p < 0.0001) and protein (p = 0.0007) yield EBVs, and hap21(TA) was significantly associated with increased SCS EBV(p = 0.0120). All significant comparison-wise associations retained significance at 8% experimental-wise level by permutation test. This study indicates that SNP c.3020A>T might play a role in the host response against mastitis and further detailed studies are needed to understand its functional mechanisms.
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影响因子：
5.4
作者：
HILL W G;ROBERTSON A
通讯作者：
ROBERTSON A
影响因子：
3.5
作者：
Kühn, C;Bennewitz, J;Kalm, E
通讯作者：
Kalm, E
DOI：
10.3758/bf03206551
发表时间：
2004-11-01
期刊：
BEHAVIOR RESEARCH METHODS INSTRUMENTS & COMPUTERS
影响因子：
--
作者：
Dunlap, WP;Xin, X;Myers, L
通讯作者：
Myers, L
影响因子：
4.9
作者：
Pierik, M;Joossens, S;Vermeire, S
通讯作者：
Vermeire, S
影响因子：
64.8
作者：
Ogura, Y;Bonen, DK;Cho, JH
通讯作者：
Cho, JH