Immunological findings in blood and bronchoalveolar lavage fluid in chronic bronchitis patients with recurrent infectious exacerbations.
Immunological findings in blood and bronchoalveolar lavage fluid in chronic bronchitis patients with recurrent infectious exacerbations.
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反复感染加重的慢性支气管炎患者血液和支气管肺泡灌洗液的免疫学发现。
DOI：
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发表时间：
1998
影响因子：
24.3
通讯作者：
B. Andersson
中科院分区：
文献类型：
--
作者：
I. Qvarfordt;G. Riise;S. Larsson;G. Almqvist;J. Rollof;T. Bengtsson;B. Andersson
支气管感染在吸烟者中很常见，似乎与慢性支气管炎（CB）的存在有关。为什么只有一些吸烟者反复发生支气管感染尚不清楚。本研究的目的是筛选与无症状吸烟者相比，CB患者和复发性感染加重患者与疾病相关的免疫变化。16例稳定的CB和复发性感染加重的吸烟者和18例无症状的吸烟者，均未经任何免疫调节治疗，接受了支气管镜检查和支气管肺泡灌洗（BAL）。吸烟史和当前吸烟状况具有可比性。测定血清免疫球蛋白（Ig）A、IgM、IgG及IgG亚类水平。分析了血液和BAL淋巴细胞表型和外周血单核细胞（PBMCs）对各种刺激物的增殖反应。测量了未刺激和破伤风类毒素刺激下PBMC细胞因子的产生。分析NK-细胞活性。白喉类毒素刺激后白喉组IgG3水平显著（p<0.05）降低，白喉类毒素刺激后白喉组PBMCs增殖反应显著（p<0.01）升高。在两个研究组培养细胞的上清液中均可检测到白细胞介素(IL)-6、肿瘤坏死因子- α (tnf - α)和干扰素- γ，但未检测到IL-2、IL-4或转化生长因子- β 2。刺激的tnf - α的产生在CB组显著升高（p<0.05）。nk细胞活性在两组间无显著差异。两组间血液或BAL淋巴细胞亚群无显著差异。总之，与无症状吸烟对照组相比，易复发性感染加重的慢性支气管炎患者的细胞介导免疫和体液免疫分析指标未发现重大变化。
Bronchial infections are common in smokers and seem to be related to the presence of chronic bronchitis (CB). Why only some smokers develop repeated bronchial infections is not known. The aim of this study was to screen for immunological changes associated with disease in patients with CB and recurrent infectious exacerbations compared to asymptomatic smokers. Sixteen smokers with stable CB and recurrent infectious exacerbations, and 18 asymptomatic smokers, all without any immunomodulating treatment, underwent bronchoscopy and bronchoalveolar lavage (BAL). Smoking history and current smoking status were comparable. Serum levels of immunoglobulin (Ig)A, IgM, IgG and IgG subclasses were measured. Blood and BAL lymphocyte phenotypes and proliferative responses of peripheral blood mononuclear cells (PBMCs) to various stimulators were analysed. Unstimulated and tetanus toxoid-stimulated production of cytokines in PBMC cultures was measured. Natural killer (NK-) cell activity was analysed. A significantly (p<0.05) lower level of IgG3 was found in the CB group, and a significantly (p<0.01) higher proliferative response of PBMCs was found in the CB group after stimulation with diphtheria toxoid. Detectable levels of interleukin (IL)-6, tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma, but not of IL-2, IL-4 or transforming growth factor-beta2, were found in supernatants from cultured cells in both study groups. Stimulated TNF-alpha production was significantly (p<0.05) higher in the CB group. NK-cell activity did not differ significantly between the study groups. There were no major differences between the groups in lymphocyte subpopulations in blood or BAL. In conclusion, no major alterations in the analysed indices of cell-mediated and humoral immunity were found in patients with chronic bronchitis prone to recurrent infectious exacerbations when compared with asymptomatic smoking controls.
DOI：
10.1164/ajrccm.150.3.8087340
发表时间：
1994-09-01
影响因子：
24.7
作者：
MCCREA, KA;ENSOR, JE;HASDAY, JD
通讯作者：
HASDAY, JD
血清免疫球蛋白E与吸烟的关系。
DOI：
10.1164/arrd.1981.124.5.523
发表时间：
1981
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Burrows,B;Halonen,M;Barbee,RA;Lebowitz,MD
通讯作者：
Lebowitz,MD
DOI：
10.1164/ajrccm.153.2.8564110
发表时间：
1996-02-01
影响因子：
24.7
作者：
deGodoy, I;Donahoe, M;Rogers, RM
通讯作者：
Rogers, RM