Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge.
Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge.
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DOI：
10.1016/j.nimb.2016.12.005
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发表时间：
2017-11-15
期刊：
影响因子：
--
通讯作者：
Wagner A
中科院分区：
文献类型：
--
作者：
Aurelius O;Duman R;El Omari K;Mykhaylyk V;Wagner A
自结构生物学开始以来，新型大分子晶体结构的定相一直具有挑战性。利用天然存在的元素（如硫和磷）的异常衍射进行定相对于某些系统是可能的，但由于必须访问更长的X射线波长以充分利用这些元素的异常散射贡献而受到阻碍。这里提出的是从第一次成功的实验相位研究的大分子晶体结构在接近硫K边缘的波长的结果。这是通过真空装置和钻石光源I23光束线上的长波长优化实验装置实现的。在这些早期调试实验中，仅应用了标准数据收集和处理程序，特别是没有使用专用的吸收校正。然而，实验的成功表明，一旦数据收集协议和数据处理得到优化，提取相位信息的能力甚至可以进一步提高。
Phasing of novel macromolecular crystal structures has been challenging since the start of structural biology. Making use of anomalous diffraction of natively present elements, such as sulfur and phosphorus, for phasing has been possible for some systems, but hindered by the necessity to access longer X-ray wavelengths in order to make most use of the anomalous scattering contributions of these elements. Presented here are the results from a first successful experimental phasing study of a macromolecular crystal structure at a wavelength close to the sulfur K edge. This has been made possible by the in-vacuum setup and the long-wavelength optimised experimental setup at the I23 beamline at Diamond Light Source. In these early commissioning experiments only standard data collection and processing procedures have been applied, in particular no dedicated absorption correction has been used. Nevertheless the success of the experiment demonstrates that the capability to extract phase information can be even further improved once data collection protocols and data processing have been optimised.
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DOI：
10.1107/s0907444913001479
发表时间：
2013-07
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Liu Q;Liu Q;Hendrickson WA
通讯作者：
Hendrickson WA
影响因子：
0.7
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Stuhrmann, HB
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
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作者：
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通讯作者：
Zwart PH
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC