miR-31 Functions as an Oncomir Which Promotes Epithelial-Mesenchymal Transition via Regulating BAP1 in Cervical Cancer.
miR-31 Functions as an Oncomir Which Promotes Epithelial-Mesenchymal Transition via Regulating BAP1 in Cervical Cancer.
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miR-31 作为 Oncomir 发挥作用，通过调节宫颈癌中的 BAP1 促进上皮间质转化
DOI：
10.1155/2017/6361420
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发表时间：
2017
影响因子：
--
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Wang N;Li Y;Zhou J
MicroRNA-31(miR-31)作为肿瘤抑制基因或参与肿瘤行为的癌基因发挥作用。然而，miR-31在宫颈癌发生中的作用尚不清楚。本研究的目的是验证miR-31和BRCA1相关蛋白-1(BAP1)在调节宫颈癌上皮-间充质转化(EMT)中的潜在作用。在本研究中，qRT-PCR检测发现miR-31在人宫颈癌细胞和临床组织中表达上调。伤口愈合和细胞迁移实验结果显示，miR-31基因的敲除抑制了细胞的转移和迁移。生物信息学和双荧光素酶报告基因分析表明，BAP1是miR-31的直接靶点。在体内移植瘤实验中，miR-31通过抑制BAP1的表达，促进了宫颈癌细胞的增殖和EMT，加速了肿瘤的生长。总体而言，这些结果强调了miR-31作为肿瘤抑制因子的重要作用，它可能通过下调BAP1的表达来促进宫颈癌的EMT。因此，miR-31的下调可能成为宫颈癌和其他恶性肿瘤分子治疗的新途径。
MicroRNA-31 (miR-31) functions as tumor suppressors or oncogenes that are involved in tumor behavior. However, the function of miR-31 in cervical carcinogenesis remains unclear. The aim of this study was to validate the potential role of miR-31 and BRCA1-associated protein-1 (BAP1) on regulating epithelial-mesenchymal transition (EMT) in cervical cancer. In the present study, qRT-PCR assay revealed that the expression of miR-31 was upregulated in human cervical cancer cells and clinical tissues. Results of wound healing and cell migration assay revealed that knockdown of miR-31 inhibited cell metastasis and migration. Bioinformatic and dual-luciferase reporter gene assay showed that BAP1 was the direct target of miR-31. Furthermore, the results revealed that miR-31 promoted proliferation and EMT in cervical cancer cells and accelerated the development of tumor growth in vivo xenograft experiment by inhibiting BAP1 expression. Overall, these results highlight an important role of miR-31 functioning as an oncomir which could promote EMT in cervical cancer via downregulating BAP1 expression. Thus, downregulation of miR-31 could be a novel approach for the molecular treatment of cervical cancers and other malignancies.
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影响因子：
11.2
作者：
Lin PC;Chiu YL;Banerjee S;Park K;Mosquera JM;Giannopoulou E;Alves P;Tewari AK;Gerstein MB;Beltran H;Melnick AM;Elemento O;Demichelis F;Rubin MA
通讯作者：
Rubin MA
DOI：
10.1126/science.1194472
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harbour JW;Onken MD;Roberson ED;Duan S;Cao L;Worley LA;Council ML;Matatall KA;Helms C;Bowcock AM
通讯作者：
Bowcock AM
BRCA1相关蛋白-1是一种需要去泛素化活性和核定位的肿瘤抑制剂。
影响因子：
11.2
作者：
Ventii KH;Devi NS;Friedrich KL;Chernova TA;Tighiouart M;Van Meir EG;Wilkinson KD
通讯作者：
Wilkinson KD
影响因子：
30.8
作者：
Wiesner, Thomas;Obenauf, Anna C.;Murali, Rajmohan;Fried, Isabella;Griewank, Klaus G.;Ulz, Peter;Windpassinger, Christian;Wackernagel, Werner;Loy, Shea;Wolf, Ingrid;Viale, Agnes;Lash, Alex E.;Pirun, Mono;Socci, Nicholas D.;Ruetten, Arno;Palmedo, Gabriele;Abramson, David;Offit, Kenneth;Ott, Arthur;Becker, Juergen C.;Cerroni, Lorenzo;Kutzner, Heinz;Bastian, Boris C.;Speicher, Michael R.
通讯作者：
Speicher, Michael R.
影响因子：
4
作者：
Castellanos JA;Merchant NB;Nagathihalli NS
通讯作者：
Nagathihalli NS