Radiosensitization Effect of Gold Nanoparticles on Plasmid DNA Damage Induced by Therapeutic MV X-rays.
Radiosensitization Effect of Gold Nanoparticles on Plasmid DNA Damage Induced by Therapeutic MV X-rays.
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DOI：
10.3390/nano12050771
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发表时间：
2022-02-25
期刊：
影响因子：
--
通讯作者：
Takami S
中科院分区：
文献类型：
--
作者：
Yogo K;Misawa M;Shimizu H;Kitagawa T;Hirayama R;Ishiyama H;Yasuda H;Kametaka S;Takami S
金纳米颗粒(AuNPs)可以与兆伏特(MV)X射线一起使用，以发挥放射增敏作用，这在细胞存活分析和小鼠实验中得到了证明。然而，具体的机制尚不清楚，除了物理剂量增加外，还提出了几种化学和生物过程。在充分增强的同时降低AuNP的浓度是AuNPs临床应用的必要条件。在这里，我们使用带正电的(+)AuNPs来确定AuNPs与MV X射线联合在体外对DNA损伤的放射增敏作用。我们研究了低浓度的AuNPs对DNA损伤和活性氧(ROS)产生的影响。1.4 nm+AuNP可促进DNA损伤，单链断裂剂量增强倍数为1.4±0.2，双链断裂剂量增强因子为1.2±0.1。+AuNPs联合MV X射线在DNA水平上诱导放射增敏，表明这种效应是物理和/或化学的。尽管−AuNPs诱导了相似的ROS水平，但它们并没有造成明显的DNA损伤。因此，低浓度+AuNPs的剂量增强可能随着DNA周围局部+AuNP浓度的增加或通过DNA结合而发生。+AUNPs比−AUNPs具有更强的放射增敏作用。+AuNPs与MV-X线联合应用于放射治疗可能会改善临床疗效。
Gold nanoparticles (AuNPs) can be used with megavolt (MV) X-rays to exert radiosensitization effects, as demonstrated in cell survival assays and mouse experiments. However, the detailed mechanisms are not clear; besides physical dose enhancement, several chemical and biological processes have been proposed. Reducing the AuNP concentration while achieving sufficient enhancement is necessary for the clinical application of AuNPs. Here, we used positively charged (+) AuNPs to determine the radiosensitization effects of AuNPs combined with MV X-rays on DNA damage in vitro. We examined the effect of low concentrations of AuNPs on DNA damage and reactive oxygen species (ROS) generation. DNA damage was promoted by 1.4 nm +AuNP with dose enhancement factors of 1.4 ± 0.2 for single-strand breaks and 1.2 ± 0.1 for double-strand breaks. +AuNPs combined with MV X-rays induced radiosensitization at the DNA level, indicating that the effects were physical and/or chemical. Although −AuNPs induced similar ROS levels, they did not cause considerable DNA damage. Thus, dose enhancement by low concentrations of +AuNPs may have occurred with the increase in the local +AuNP concentration around DNA or via DNA binding. +AuNPs showed stronger radiosensitization effects than −AuNPs. Combining +AuNPs with MV X-rays in radiation therapy may improve clinical outcomes.
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影响因子：
8
作者：
Yogo K;Misawa M;Shimizu M;Shimizu H;Kitagawa T;Hirayama R;Ishiyama H;Furukawa T;Yasuda H
通讯作者：
Yasuda H
影响因子：
3.8
作者：
Almond, PR;Biggs, PJ;Rogers, DWO
通讯作者：
Rogers, DWO
影响因子：
3.7
作者：
Yogo K;Ogawa T;Hayashi M;Harada Y;Nishizaka T;Kinosita K Jr
通讯作者：
Kinosita K Jr
影响因子：
2.6
作者：
Hanai, R;Yazu, M;Hieda, K
通讯作者：
Hieda, K
DOI：
10.1016/j.nano.2013.04.007
发表时间：
2013-10-01
影响因子：
5.4
作者：
Bobyk, Laure;Edouard, Magali;Elleaume, Helene
通讯作者：
Elleaume, Helene