Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer.
Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer.
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线粒体 DNA 含量可能不是单倍体囊胚移植后活产的可靠筛选生物标志物
DOI：
10.3389/fendo.2021.762976
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发表时间：
2021
影响因子：
5.2
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Zhou X;Liu X;Shi W;Ye M;Chen S;Xu C
越来越多的研究将线粒体 DNA (mtDNA) 含量与胚胎活力和移植结果联系起来。但以往的研究更多关注mtDNA与胚胎着床的关系，很少有研究研究mtDNA含量对活产的影响。在这项研究中，我们调查了 mtDNA 含量是否是单囊胚移植后活产的可靠筛查生物标志物。该研究共纳入233对夫妇，拥有316个囊胚期胚胎，接受体外受精治疗和植入前基因检测分析。所有胚胎染色体均正常，并接受过单胚胎移植。活产组、流产组和非着床组囊胚mtDNA含量无显着差异（p=0.999），流产组和活产组囊胚mtDNA含量相似[中位数（四分位距），1.00*108（7.59*107- 1.39*108）vs 1.01*108 (7.37*107-1.32*108)]。同样，mtDNA 含量和胚胎着床潜力之间没有观察到显着相关性 (p=0.965)。使用广义估计方程调整逻辑回归分析中的多个混杂因素后，在所有囊胚、第 5 天和第 6 天囊胚中未观察到 mtDNA 含量与活产之间的关联（分别为 p=0.567、p=0.673、p=0.165）。 mtDNA 含量升高的囊胚和 mtDNA 含量正常的囊胚之间的活产率没有显着差异（26.7% vs 33.6% p=0.780）。此外，mtDNA 含量与母亲年龄之间不存在线性相关性（p=0.570）。总之，根据现有的测试工具，线粒体DNA含量似乎并不是胚胎移植结果（即植入和活产）的潜在生物标志物。线粒体 DNA 含量升高的胚胎也具有成功活产的发育潜力。
An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth. In the study, we investigated whether mtDNA content is a reliable screening biomarker for live birth after single blastocyst transfer. A total of 233 couples with 316 blastocyst stage embryos undergoing in vitro fertilization treatment and pre-implantation genetic testing analysis were included in the study. All embryos were chromosomally normal and had undergone single-embryo transfers. There was no significant difference observed in the blastocyst mtDNA content among the live birth, miscarriage and non-implanted groups (p=0.999), and the mtDNA content in blastocysts from the miscarriage and live birth groups was similar [median (interquartile range), 1.00*108(7.59*107- 1.39*108) vs 1.01*108 (7.37*107- 1.32*108)]. Similarly, no significant association was observed between mtDNA content and embryo implantation potential (p=0.965). After adjusting for multiple confounders in a logistic regression analysis with generalized estimating equations, no associations between mtDNA content and live birth were observed in all blastocysts, Day-5 and Day-6 blastocysts (p=0.567, p=0.673, p=0.165, respectively). The live birth rate was not significantly different between blastocysts with an elevated mtDNA content and blastocysts with a normal mtDNA content (26.7% vs 33.6% p=0.780). Additionally, there was no linear correlation between the mtDNA content and maternal age (p=0.570). In conclusion, the mtDNA content does not seem to be a potential biomarker for embryo transfer outcomes (i.e., implantation and live birth) based on the existing testing tools. Embryos with an elevated mtDNA content also have development potential for successful live birth.
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影响因子：
4.5
作者：
Fragouli E;Spath K;Alfarawati S;Kaper F;Craig A;Michel CE;Kokocinski F;Cohen J;Munne S;Wells D
通讯作者：
Wells D
影响因子：
6.7
作者：
Fischer, Jill;Colls, Pere;Munne, Santiago
通讯作者：
Munne, Santiago
影响因子：
6.1
作者：
Fragouli, Elpida;McCaffrey, Caroline;Wells, Dagan
通讯作者：
Wells, Dagan
影响因子：
--
作者：
Kirillova, Anastasia;Lysenkov, Sergey;Sukhikh, Gennady
通讯作者：
Sukhikh, Gennady
DOI：
10.1042/bcj20190767
发表时间：
2020-11-13
期刊：
The Biochemical journal
影响因子：
--
作者：
Hock DH;Robinson DRL;Stroud DA
通讯作者：
Stroud DA