Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.
Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.
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DOI：
10.32598/bcn.2021.973.3
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发表时间：
2022-09
影响因子：
1.7
通讯作者：
Elyasi, Leila
中科院分区：
文献类型：
--
作者：
Moayeri, Ardeshir;Alizadeh, Rafieh;Hamidabadi, Hatef Ghasemi;Bojnordi, Maryam Nazm;Niapour, Ali;Hedayatpour, Azim;Darvishi, Marzieh;Heidari, Fatemeh;Soleimani, Maryam;Elyasi, Leila
人脐带间充质干细胞（HUC-MSCs）向多巴胺能神经元的诱导是组织工程和各种神经退行性疾病（包括帕金森病）的实验和临床治疗中的主要挑战。本研究旨在诱导HUC-MSCs分化为多巴胺能神经元样细胞。在HUC-MSC的分离和表征之后，将它们转移到Matrigel包被的板上，并与多巴胺能神经元分化因子的混合物一起孵育。通过实时聚合酶链反应、免疫细胞化学和高效液相色谱法评估了在二维培养和基质胶上分化为多巴胺能神经元样细胞的能力。我们的研究结果表明，与2D培养板相比，在Matrigel分化的细胞上多巴胺能神经元标记物的转录和蛋白水平显著增加。总的来说，本研究的结果表明，HUC-MSCs可以成功地分化为多巴胺能神经元样细胞的基质胶，具有治疗多巴胺能神经元相关疾病的巨大潜力。
The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells. Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography. Our results showed that dopaminergic neuronal markers' transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates. Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
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影响因子：
64.8
作者：
Kriks, Sonja;Shim, Jae-Won;Piao, Jinghua;Ganat, Yosif M.;Wakeman, Dustin R.;Xie, Zhong;Carrillo-Reid, Luis;Auyeung, Gordon;Antonacci, Chris;Buch, Amanda;Yang, Lichuan;Beal, M. Flint;Surmeier, D. James;Kordower, Jeffrey H.;Tabar, Viviane;Studer, Lorenz
通讯作者：
Studer, Lorenz
影响因子：
5.3
作者：
Åkerud, P;Canals, JM;Arenas, E
通讯作者：
Arenas, E
影响因子：
2.9
作者：
Koh, Seong-Ho;Kim, Kyung Suk;Kim, Seung Hyun
通讯作者：
Kim, Seung Hyun
影响因子：
3
作者：
Aligholi, Hadi;Hassanzadeh, Gholamreza;Gorji, Ali
通讯作者：
Gorji, Ali
临床级人脐带间充质干细胞通过改善突触可塑性和内源性神经发生来逆转认知衰老
影响因子：
9
作者：
Cao N;Liao T;Liu J;Fan Z;Zeng Q;Zhou J;Pei H;Xi J;He L;Chen L;Nan X;Jia Y;Yue W;Pei X
通讯作者：
Pei X