Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart.
Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart.
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DOI：
10.1242/dev.152124
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发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Vermot J
中科院分区：
文献类型：
--
作者：
Boselli F;Steed E;Freund JB;Vermot J
心肌收缩力和血流为心脏的形态发生提供了重要的机械线索。一般来说，内皮细胞根据流动方向性响应剪切应力模式而改变其迁移行为。在这里，我们评估了剪切应力模式和流动方向性对心内膜细胞（心脏的特殊内皮细胞）行为的影响。在斑马鱼心脏瓣膜形成的早期阶段，我们发现心内膜细胞正在汇聚到瓣膜形成区域，并且这种行为取决于机械力。定量实时成像和数学建模使我们能够将这种组织收敛与潜在的流动力关联起来。我们预测组织收敛与平均壁剪切应力和谐波相平均剪切应力梯度的方向相关，令人惊讶的是，它们与流动的总体方向不匹配。这与血流方向性在血管发育中的通常作用形成对比，表明在研究体内内皮细胞对血流的反应时，应考虑壁剪切应力的完整空间和时间复杂性。摘要：血流模型表明，动态剪切应力模式（而不是平均血流方向）可以预测心脏瓣膜形成早期阶段心内膜细胞的典型行为。
Myocardial contractility and blood flow provide essential mechanical cues for the morphogenesis of the heart. In general, endothelial cells change their migratory behavior in response to shear stress patterns, according to flow directionality. Here, we assessed the impact of shear stress patterns and flow directionality on the behavior of endocardial cells, the specialized endothelial cells of the heart. At the early stages of zebrafish heart valve formation, we show that endocardial cells are converging to the valve-forming area and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase-averaged shear stresses, which surprisingly do not match the overall direction of the flow. This contrasts with the usual role of flow directionality in vascular development and suggests that the full spatial and temporal complexity of the wall shear stress should be taken into account when studying endothelial cell responses to flow in vivo. Summary: Blood flow modeling shows that dynamic shear stress patterns, rather than mean flow direction, predict the stereotypical behavior of endocardial cells during the early steps of heart valve formation.
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11.8
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