Actin-Microtubule Crosstalk Imparts Stiffness to the Contractile Ring in Fission Yeast.
Actin-Microtubule Crosstalk Imparts Stiffness to the Contractile Ring in Fission Yeast.
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肌动蛋白-微管相互作用对于细胞分裂至关重要，但这些聚合物网络如何相互影响它们在活细胞中的机械性能和功能仍然未知。在裂殖酵母中，微管的后期阵列 (PAA) 在收缩环平面中组装，其组装依赖于 Myp2p 依赖性 Mto1p 的募集，Mto1p 是赤道微管组织中心 (eMTOC) 的组成部分。该微管阵列的一般组织及其对收缩环物理附着的影响仍不清楚。我们发现 Myp2p 促进 Mto1p 募集到收缩环的内表面，其中 eMTOC 在不直接相互作用的情况下聚合微管。 PAA 微管在收缩环内形成 Ase1p 交联微管的动态多边形。 PAA 微管的特定损失通过降低肌动蛋白收缩环的刚度来影响其机械性能。环机械性能的这种变化对胞质分裂或环的锚定没有可测量的影响。我们的工作表明，PAA 微管在细胞分裂过程中利用收缩环进行组装和发挥功能，而收缩环可能不会从这些相互作用中获益。
Actin–microtubule interactions are critical for cell division, yet how these networks of polymers mutually influence their mechanical properties and functions in live cells remains unknown. In fission yeast, the post-anaphase array (PAA) of microtubules assembles in the plane of the contractile ring, and its assembly relies on the Myp2p-dependent recruitment of Mto1p, a component of equatorial microtubule organizing centers (eMTOCs). The general organization of this array of microtubules and the impact on their physical attachment to the contractile ring remain unclear. We found that Myp2p facilitates the recruitment of Mto1p to the inner face of the contractile ring, where the eMTOCs polymerize microtubules without their direct interaction. The PAA microtubules form a dynamic polygon of Ase1p crosslinked microtubules inside the contractile ring. The specific loss of PAA microtubules affects the mechanical properties of the contractile ring of actin by lowering its stiffness. This change in the mechanical properties of the ring has no measurable impact on cytokinesis or on the anchoring of the ring. Our work proposes that the PAA microtubules exploit the contractile ring for their assembly and function during cell division, while the contractile ring may receive no benefit from these interactions.
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