Seroepidemiological study of human herpesvirus‐6 and ‐7 in children of different ages and detection of these two viruses in throat swabs by polymerase chain reaction
Seroepidemiological study of human herpesvirus‐6 and ‐7 in children of different ages and detection of these two viruses in throat swabs by polymerase chain reaction
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不同年龄段儿童人类疱疹病毒‐6和‐7的血清流行病学研究及咽拭子中两种病毒的聚合酶链反应检测
DOI：
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发表时间：
1996
影响因子：
12.7
通讯作者：
K. Yamanishi
中科院分区：
文献类型：
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作者：
K. Tanaka;T. Kondo;T. Mukai;H. Miyoshi;Y. Yamamoto;S. Okada;K. Yamanishi
采用聚合酶链反应（PCR）检测62例不同年龄组（Ⅰ组0-5月龄、Ⅱ组6-11月龄、Ⅲ组12-23月龄、Ⅳ组28岁）儿童和28例成人咽拭子中人类疱疹病毒6型（HHV-6）和人类疱疹病毒7型（HHV-7）的存在。HHV-6 DNA的检出率在1岁儿童中最高（87%），并随年龄的增长而下降，而HHV-7的检出率随年龄的增长而增加，并在成人中达到最大值。除了两名仅分泌HHV-6A的儿童外，几乎所有样本中都检测到HHV-6 B。4组儿童抗体阳性率分别为8/12（66.7%）、10/12（83.3%）、15/16（93.8%）、13/14（92.9%）。HHV-7抗体检出率分别为6/12（50.0%）、4/12（33.3%）、12/16（75.0%）和13/14（92.9%）。通过使用在第一名患者发病后从这些儿童中顺序收集的样本，尝试检测连续发生皮疹的三胞胎咽拭子中的HHV-6 DNA。在所有患者的急性期和恢复期均可检测到高拷贝数的HHV-6 DNA，但在发病前采集的样本中未检测到DNA。© 1996 Wiley利斯公司
The presence of human herpesvirus 6 (HHV‐6) and human herpesvirus 7 (HHV‐7) in throat swabs of 62 children of different age groups (group I, ages 0–5 month; group II, ages 6–11 months, group III, ages 12–23 months, group IV, age 2–8 years) and 28 adults was detected by polymerase chain reaction. The detection rate of HHV‐6 DNA was the highest (87%) in children aged 1‐year‐old and decreased with age, whereas the detection rate of HHV‐7 increased with age and reached a maximum in adults. HHV‐6B was detected in almost all samples except for two children who secreted only HHV‐6A. When the antibody prevalence was determined in the four groups of children, HHV‐6 antibody was detected in 8/12 (66.7%), 10/12 (83.3%), 15/16 (93.8%), and 13/14 (92.9%), respectively. Antibody to HHV‐7 in these groups was detected in 6/12 (50.0%), 4/12 (33.3%), 12/16 (75.0%), and 13/14 (92.9%), respectively. Detection of HHV‐6 DNA in throat swabs of triplets who had the sequential onset of exanthem subitum was attempted by using samples sequentially collected from these children after the onset of the disease in the first patient. HHV‐6 DNA with high copy numbers was detectable during the acute and convalescent phases of the disease in all patients, but no DNA was detected in samples collected before the onset of disease. © 1996 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.89.21.10552
发表时间：
1992-11-01
影响因子：
11.1
作者：
BERNEMAN, ZN;ABLASHI, DV;GALLO, RC
通讯作者：
GALLO, RC