Immunomodulatory Mediators from Pollen Enhance the Migratory Capacity of Dendritic Cells and License Them for Th2 Attraction1
Immunomodulatory Mediators from Pollen Enhance the Migratory Capacity of Dendritic Cells and License Them for Th2 Attraction1
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花粉中的免疫调节介质增强树突状细胞的迁移能力并允许它们吸引 Th21
DOI：
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发表时间：
2007
影响因子：
4.4
通讯作者：
C. Traidl‐Hoffmann
中科院分区：
文献类型：
--
作者：
V. Mariani;S. Gilles;T. Jakob;M. Thiel;Martin J. Mueller;J. Ring;H. Behrendt;C. Traidl‐Hoffmann
特应性个体对过敏原的免疫应答的特征在于Th 2细胞因子和趋化因子水平的增加。然而，细胞因子/趋化因子谱与入侵过敏原类型相匹配的方式，以及为什么这些谱有时会脱轨并导致疾病，还没有得到很好的理解。我们最近证明，花粉调节树突状细胞（DC）功能的方式，导致在体外启动Th 2反应的能力增强。在这里，我们研究了桦树花粉水提取物（Bet.- APE）对人单核细胞衍生DC的趋化因子受体表达和趋化因子产生的影响。打赌APE强烈诱导CXCR 4的表达和功能，并降低CCR 1和CCR 5在未成熟DC上的表达。此外，用Bet.- APE显著降低LPS诱导的CXCL 10/IP-10、CCL 5/RANTES的产生;诱导CCL 22/巨噬细胞衍生的趋化因子;并且不显著改变CCL 17/胸腺和活化调节趋化因子的释放。在功能层面上，打赌。APE增加LPS刺激的DC吸引Th 2细胞的能力，而募集Th 1细胞的能力降低。打赌APE显著地和剂量依赖性地增强细胞内cAMP，表明来自花粉粒的水溶性因子结合Gα s蛋白偶联受体。E1-Phytoprostanes被确定为是一个球员在水花粉提取物的Th 2极化潜力。总之，我们的研究结果表明，花粉本身释放调节介质，产生一个Th 2促进微环境与优先招聘的Th 2细胞的花粉暴露的网站。
The immune response of atopic individuals against allergens is characterized by increased levels of Th2 cytokines and chemokines. However, the way in which the cytokine/chemokine profile is matched to the type of invading allergen, and why these profiles sometimes derail and lead to disease, is not well understood. We recently demonstrated that pollen modulates dendritic cell (DC) function in a way that results in an enhanced capacity to initiate Th2 responses in vitro. Here, we examined the effects of aqueous birch pollen extracts (Bet.-APE) on chemokine receptor expression and chemokine production by human monocyte-derived DCs. Bet.-APE strongly induced expression and function of CXCR4 and reduced CCR1 and CCR5 expression on immature DCs. In addition, DC treatment with Bet.-APE significantly reduced LPS-induced production of CXCL10/IP-10, CCL5/RANTES; induced CCL22/macrophage-derived chemokine; and did not significantly change release of CCL17/thymus and activation-regulated chemokine. At a functional level, Bet.-APE increased the capacity of LPS-stimulated DCs to attract Th2 cells, whereas the capacity to recruit Th1 cells was reduced. Bet.-APE significantly and dose-dependently enhanced intracellular cAMP, suggesting that water-soluble factors from pollen grains bind a Gαs-protein-coupled receptor. E1-Phytoprostanes were identified to be one player in the Th2-polarizing potential of aqueous pollen extracts. In summary, our results demonstrate that pollen itself releases regulatory mediators which generate a Th2-promoting micromilieu with preferential recruitment of Th2 cells to the site of pollen exposure.
小鼠系膜细胞中 RANTES 和 ICAM-1 表达的调节。
DOI：
10.1681/asn.v84596
发表时间：
1997
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Satriano,JA;Banas,B;Luckow,B;Nelson,P;Schlondorff,DO
通讯作者：
Schlondorff,DO
cAMP 上调淋巴细胞 CXCR4 的细胞表面表达：对趋化性和 HIV-1 感染的影响。
DOI：
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发表时间：
1999
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Cole,SW;Jamieson,BD;Zack,JA
通讯作者：
Zack,JA
DOI：
10.1084/jem.184.3.1101
发表时间：
1996-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bleul CC;Fuhlbrigge RC;Casasnovas JM;Aiuti A;Springer TA
通讯作者：
Springer TA