Design and Synthesis of Bovine Leukemia Virus-Associated Peptide-Based Qβ Conjugate Eliciting Long-Lasting Neutralizing Antibodies in Mice.
Design and Synthesis of Bovine Leukemia Virus-Associated Peptide-Based Qβ Conjugate Eliciting Long-Lasting Neutralizing Antibodies in Mice.
复制标题
DOI：
10.1021/acsinfecdis.2c00001
复制
发表时间：
2022-05-13
影响因子：
5.3
通讯作者：
Huang, Xuefei
中科院分区：
文献类型：
--
作者：
Chugh, Shivangi;Swenson, Cheryl;Yuzbasiyan-Gurkan, Vilma;Huang, Xuefei
牛白血病病毒（BLV）是一种牛c型逆转录病毒，在世界上大多数国家，随着感染率的上升，造成了巨大的经济损失。为了开发抗blv疫苗，我们利用包膜糖蛋白gp51肽表位（一个假定的受体结合位点）构建了肽偶联物。这种高抗原性肽通过两种不同的连接策略，异硫氰酸酯（NCS）和己二硝基苯酯（二硝基苯）与突变噬菌体载体（mQβ）共价连接。两种构建物均比相应的锁孔帽贝血青素蛋白载体（金标准）在ncs连接体策略下的小鼠中获得更高的抗blv肽IgG滴度，需要较少的样品处理。mq - β-gp51肽构建物是首个产生持久免疫（bbb539天）的基于BLV肽的候选疫苗，它能识别天然gp51蛋白和BLV颗粒，并显著降低暴露于感染性BLV的易感细胞系的融合。这些结果支持该疫苗结构具有很高的转化和动物健康潜力。
Bovine leukemia virus (BLV) is a C-type retrovirus of cattle that causes huge economic losses with the infection rate escalated in the majority of countries worldwide. To develop an anti-BLV vaccine, we constructed a peptide-conjugate using the envelope glycoprotein gp51 peptide-epitope, a putative receptor-binding site. This highly antigenic peptide was covalently linked to a mutant bacteriophage carrier (mQβ) using two different linker strategies, isothiocyanate (NCS) and dinitrophenyl adipate. Both constructs elicited higher anti-BLV peptide IgG titers than the corresponding conjugate with keyhole limpet hemocyanin protein carrier (gold standard) in mice with the NCS-linker strategy requiring less sample processing. The mQβ-gp51-peptide construct is the first BLV peptide-based vaccine candidate to generate durable immunity (>539 days), which recognized both native gp51 protein and BLV particles, and significantly decreased fusion of a susceptible cell line exposed to infectious BLV. These results support the high translational and animal health potential of the vaccine construct.
登录
查看更多内容
影响因子：
4
作者：
Sungsuwan, Suttipun;Wu, Xuanjun;Shaw, Vincent;Kavunja, Herbert;McFall-Boegeman, Hunter;Rashidijahanabad, Zahra;Tan, Zibin;Lang, Shuyao;Nick, Setare Tahmasebi;Lin, Po-han;Yin, Zhaojun;Ramadan, Sherif;Jin, Xiangshu;Huang, Xuefei
通讯作者：
Huang, Xuefei
影响因子：
3.7
作者：
Rola-Łuszczak M;Pluta A;Olech M;Donnik I;Petropavlovskiy M;Gerilovych A;Vinogradova I;Choudhury B;Kuźmak J
通讯作者：
Kuźmak J
影响因子：
7.8
作者：
Guo, Zhongwu;Wang, Qianli
通讯作者：
Wang, Qianli
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
3.3
作者：
Trono, KG;Mariano, D;Carrillo, C
通讯作者：
Carrillo, C