Proteomic analysis of immediate-early response plasma proteins after 70% and 90% partial hepatectomy.
Proteomic analysis of immediate-early response plasma proteins after 70% and 90% partial hepatectomy.
复制标题
70％和90％部分肝切除术后立即反应血浆蛋白的蛋白质组学分析。
DOI：
10.1111/hepr.12030
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Dai G
中科院分区：
文献类型：
--
作者：
Kumar S;Zou Y;Bao Q;Wang M;Dai G
部分肝切除术（PH）诱导强大的肝脏再生和代谢反应，被认为是由体液因素引发的。该研究的目的是确定可能触发或反映身体对肝脏质量减少的立即早期反应的血浆蛋白因子。对雄性C57 BL/6小鼠进行假手术、70%PH或90%PH。在手术后20、60和180分钟从下腔静脉收集血液。使用无标记的定量质谱为基础的蛋白质组学方法，我们确定了399蛋白质表现出显着的变化，在任何两组之间的血浆表达。在399种蛋白质中，167种蛋白质具有多个独特序列和高肽ID置信度（>90%），并被定义为优先级1的蛋白质。一组主要与代谢相关的血浆蛋白在70%PH后富集。在对90%PH应答的血浆蛋白中，有一组主要蛋白也与代谢和一种已知的细胞因子（血小板因子4）相关。90% PH和70% PH诱导血浆蛋白谱的相似变化。我们的研究结果使我们能够深入了解血浆蛋白对肝脏质量损失的即时早期反应。我们的数据支持这样一种观点，即在大量肝脏质量损失后，身体代谢需求的增加可能起到校准肝脏再生反应的传感器的作用。
Partial hepatectomy (PH) induces robust hepatic regenerative and metabolic responses that are considered to be triggered by humoral factors. The aim of the study was to identify plasma protein factors that potentially trigger or reflect the body’s immediate-early responses to liver mass reduction. Male C57BL/6 mice were subjected to sham operation, 70% PH, or 90% PH. Blood was collected from the inferior vena cava at 20, 60, and 180 minutes after surgery. Using a label-free quantitative mass spectrometry-based proteomics approach, we identified 399 proteins exhibiting significant changes in plasma expression between any two groups. Of the 399 proteins, 167 proteins had multiple unique sequences and high peptide ID confidence (>90%) and were defined as priority 1 proteins. A group of plasma proteins largely associated with metabolism is enriched after 70% PH. Among the plasma proteins that respond to 90% PH are a dominant group of proteins that are also associated with metabolism and one known cytokine (platelet factor 4). Ninety percent PH and 70% PH induces similar changes in plasma protein profile. Our findings enable us to gain insight into the immediate-early response of plasma proteins to liver mass loss. Our data support the notion that increased metabolic demands of the body after massive liver mass loss may function as a sensor that calibrates hepatic regenerative response.
登录
查看更多内容
DOI：
10.1016/j.jnutbio.2010.01.005
发表时间：
2010-11
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Atshaves BP;Martin GG;Hostetler HA;McIntosh AL;Kier AB;Schroeder F
通讯作者：
Schroeder F
影响因子：
13.5
作者：
Fausto, N;Campbell, JS;Riehle, KJ
通讯作者：
Riehle, KJ
影响因子：
4.4
作者：
Higgs, Richard E.;Knierman, Michael D.;Hale, John E.
通讯作者：
Hale, John E.
影响因子：
1.9
作者：
Greene, AK;Puder, M
通讯作者：
Puder, M
影响因子：
4.6
作者：
Lehmann, TG;Luedde, T;Brenner, DA
通讯作者：
Brenner, DA