Comparison of three commercial RT-PCR systems for the detection of respiratory viruses.
Comparison of three commercial RT-PCR systems for the detection of respiratory viruses.
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DOI：
10.1016/j.jcv.2014.08.010
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Stellrecht KA
中科院分区：
文献类型：
--
作者：
Butt SA;Maceira VP;McCallen ME;Stellrecht KA
We evaluated FilmArray RP and Verigene RV+ with Prodesse for respiratory viruses. Prodesse had the lowest limit of detection for all FluA strains, PIV1, PIV2 and AdV. RV+ had the lowest LOD for FluB; and RP had the lowest LOD for RSV, PIV3 and hMPV. Clinical sensitivities for influenza A, B and RSV were 100% and 98% for RV+ and RP. Due to the insensitivity of rapid tests for respiratory viruses, nucleic acid amplification tests are quickly becoming the standard of care. The performance of the FilmArray Respiratory Panel (RP) and Verigene RV+ (RV+) were compared in a retrospective analysis of 89 clinical specimens previously determined to be positive for the following viruses by our test of record, Prodesse (Pro): influenza A (29, FluA), influenza B (13, FluB), respiratory syncytial virus (12, RSV), human metapneumovirus (10, hMPV), parainfluenza (14, PIV), and adenovirus (10, AdV). Samples positive for influenza A, B or RSV were tested by both methods, while the remainder were tested by RP only. True positives were defined as positive by two or more assays. Limit of detection (LOD) analyses demonstrated Pro had the lowest LOD for all FluA strains tested, PIV1, PIV2 and AdV; RV+ had the lowest LOD for FluB; and RP had the lowest LOD for RSV, PIV3 and hMPV. Of the 55 samples tested by RV+, all 54 true positive samples were positive by RV+. Of the 89 samples tested by RP, 85 of the 88 true positive samples were positive by RP. From these results, the overall sensitivities for influenza A, B and RSV were 100% and 98% for RV+ and RP, respectively. The overall sensitivity of RP for all viruses was 97%. In summary, these systems demonstrated excellent performance. Furthermore, each system has benefits which will ensure they will all have a niche in a clinical laboratory.
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影响因子：
120.7
作者：
Thompson, WW;Shay, DK;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
8.8
作者：
Ginocchio, Christine C.;Lotlikar, Madhavi;Manji, Ryhana
通讯作者：
Manji, Ryhana
影响因子：
9.4
作者：
Jannetto, Paul J.;Buchan, Blake W.;Ledeboer, Nathan A.
通讯作者：
Ledeboer, Nathan A.
影响因子：
9.4
作者：
Novak-Weekley, S. M.;Marlowe, E. M.;Robinson, C. C.
通讯作者：
Robinson, C. C.
影响因子：
24.3
作者：
Jansen, A. G. S. C.;Sanders, E. A. M.;Hak, E.
通讯作者：
Hak, E.