Effects of UVR and UVR‐induced Cytokines on Production of Extracellular Matrix Proteins and Proteases by Dermal Fibroblasts Cultured in Collagen Gels ¶
Effects of UVR and UVR‐induced Cytokines on Production of Extracellular Matrix Proteins and Proteases by Dermal Fibroblasts Cultured in Collagen Gels ¶
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UVR 和 UVR 诱导的细胞因子对胶原凝胶中培养的真皮成纤维细胞产生细胞外基质蛋白和蛋白酶的影响 ¶
DOI：
10.1111/j.1751-1097.2004.tb09861.x
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发表时间：
2004
影响因子：
3.3
通讯作者：
I. Kochevar
中科院分区：
文献类型：
--
作者：
S. Kossodo;W. Wong;G. Simon;I. Kochevar
细胞外基质(ECM)蛋白的合成及其被基质金属蛋白酶(MMPs)的降解是皮肤长期暴露于紫外线辐射(UVR)引起的真皮重塑的一部分。我们比较了这些反应的两种替代机制，即UV-B或UV-A被成纤维细胞吸收的直接机制和间接机制，即皮肤对UVR的反应产生的细胞因子刺激ECM蛋白和基质金属蛋白酶的产生。这些研究是在人的真皮成纤维细胞上进行的，这些细胞生长在收缩的、自由漂浮的9天大的胶原蛋白凝胶中，作为真皮的等效物。测定原弹性蛋白、胶原、纤维蛋白、基质金属蛋白酶-1、-2、-3、-9及金属蛋白酶组织抑制物-1、-2的合成。原弹性蛋白、胶原和纤维蛋白水平在第4~10天保持稳定，而基质金属蛋白酶和组织金属蛋白酶在第10天下降。UV-B(2.5-50MJ/cm2)和UV-A(2-12J/cm2)均不影响ECM蛋白的合成，但UV-A可促进基质金属蛋白酶-1和-3的合成。转化生长因子-β1(5 ng/mL)处理后，原弹性蛋白合成增加。IL-1RNA和肿瘤坏死因子-β(10 ng/mL)均显著降低纤维蛋白原信使α水平，增加MMP1、MMP3和MMP9的合成。UV-B、UV-A或细胞因子对胶原合成无明显影响。这些结果表明，某些细胞因子对真皮中培养的成纤维细胞产生ECM蛋白和基质金属蛋白酶的影响可能大于对UV-B和UV-A的吸收，提示前者可能在光老化皮肤的真皮重塑中发挥作用。
Synthesis of extracellular matrix (ECM) proteins and their degradation by matrix metalloproteinases (MMP) are part of the dermal remodeling resulting from chronic exposure of skin to ultraviolet radiation (UVR). We have compared two alternative mechanisms for these responses, namely, a direct mechanism in which UV‐B or UV‐A is absorbed by fibroblasts and an indirect mechanism in which cytokines, produced in skin in response to UVR, stimulate production of the ECM proteins and MMP. These studies were carried out on human dermal fibroblasts grown in contracted, free‐floating 9 day old collagen gels as a dermal equivalent. Synthesis of tropoelastin, collagen, fibrillin, MMP‐1, ‐2, ‐3 and ‐9 and tissue inhibitors of metalloproteinases (TIMP)‐1 and ‐2 were measured. Tropoelastin, collagen and fibrillin levels were stable between days 4 and 10, and MMP and TIMP decreased by day 10. Neither UV‐B (2.5–50 mJ/cm2) nor UV‐A (2–12 J/cm2) altered synthesis of ECM proteins, but UV‐A increased MMP‐1 and ‐3 production. Tropoelastin synthesis increased in response to transforming growth factor‐β1 (5 ng/mL) treatment. Both interleukin‐1β and tumor necrosis factor‐α (10 ng/mL) decreased fibrillin messenger RNA levels but increased MMP‐1, ‐3 and ‐9 synthesis markedly. Collagen synthesis was not modulated by UV‐B, UV‐A or cytokine treatment. These results indicate that certain cytokines may have greater effects on production of ECM proteins and MMP than absorption of UV‐B and UV‐A by fibroblasts grown in dermal equivalents and suggest that the former pathway may play a role in the dermal remodeling in photoaged skin.
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多功能蛋白聚糖和核心蛋白聚糖基因在光老化和防晒皮肤中的差异表达。
DOI：
--
发表时间：
1995
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Bernstein,EF;Fisher,LW;Li,K;LeBaron,RG;Tan,EM;Uitto,J
通讯作者：
Uitto,J
影响因子：
6.4
作者：
U. Kucich;J. Rosenbloom;W. Abrams;J. Rosenbloom
通讯作者：
U. Kucich;J. Rosenbloom;W. Abrams;J. Rosenbloom
影响因子：
6.5
作者：
BERNSTEIN, EF;BROWN, DB;UITTO, J
通讯作者：
UITTO, J
影响因子：
6.5
作者：
PETERSEN, MJ;HANSEN, C;CRAIG, S
通讯作者：
CRAIG, S
影响因子：
6.5
作者：
BERNSTEIN, EF;CHEN, YQ;UITTO, J
通讯作者：
UITTO, J