An approach for quantitative mapping of synaptic periactive zone architecture and organization.
An approach for quantitative mapping of synaptic periactive zone architecture and organization.
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DOI：
10.1091/mbc.e22-08-0372
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发表时间：
2023-05-15
影响因子：
3.3
通讯作者：
Rodal, Avital A.
中科院分区：
文献类型：
--
作者：
Del Signore, Steven J.;Mitzner, Margalit G.;Silveira, Anne M.;Fai, Thomas G.;Rodal, Avital A.
在活性区发生胞吐后，突触囊泡膜和膜结合蛋白必须被循环利用。驱动这种循环的内吞机制在临近活跃区（PAZ）的突触区域积累，但该机制在PAZ内的组织以及PAZ成分与活跃区释放特性的关系尚不清楚。PAZ也富含细胞粘附蛋白，但它们在这些位点的功能尚不清楚。在这里，我们利用果蝇突触的airscan和受激发射损耗成像，建立了一个描述PAZ组织和超微结构的定量框架。不同的内吞蛋白定位于PAZ的不同区域，表明亚结构域专门用于不同的生化活动、膜重塑阶段或突触功能。我们发现，内吞性而非粘附性PAZ蛋白的积累和分布与活性区支架蛋白Bruchpilot的丰度相关，这是一种释放概率的结构相关。这些数据表明，内吞和胞吐活动具有空间相关性。综上所述，我们的研究结果确定了突触中胞外和内吞装置之间的新关系，并为量化突触结构提供了一个新的概念框架。
Following exocytosis at active zones, synaptic vesicle membranes and membrane-bound proteins must be recycled. The endocytic machinery that drives this recycling accumulates in the periactive zone (PAZ), a region of the synapse adjacent to active zones, but the organization of this machinery within the PAZ, and how PAZ composition relates to active zone release properties, remains unknown. The PAZ is also enriched for cell adhesion proteins, but their function at these sites is poorly understood. Here, using Airyscan and stimulated emission depletion imaging of Drosophila synapses, we develop a quantitative framework describing the organization and ultrastructure of the PAZ. Different endocytic proteins localize to distinct regions of the PAZ, suggesting that subdomains are specialized for distinct biochemical activities, stages of membrane remodeling, or synaptic functions. We find that the accumulation and distribution of endocytic but not adhesion PAZ proteins correlate with the abundance of the scaffolding protein Bruchpilot at active zones—a structural correlate of release probability. These data suggest that endocytic and exocytic activities are spatially correlated. Taken together, our results identify novel relationships between the exocytic and endocytic apparatus at the synapse and provide a new conceptual framework to quantify synaptic architecture.
影响因子：
5.3
作者：
Dagar S;Teng Z;Gottmann K
通讯作者：
Gottmann K
突触囊泡具有两个不同的回收途径。
影响因子：
7.8
作者：
Koenig, JH;Ikeda, K
通讯作者：
Ikeda, K