Synaptic vesicles have two distinct recycling pathways.
Synaptic vesicles have two distinct recycling pathways.
复制标题
突触囊泡具有两个不同的回收途径。
DOI：
10.1083/jcb.135.3.797
复制
发表时间：
1996-11
影响因子：
7.8
通讯作者：
Ikeda, K
中科院分区：
文献类型：
--
作者：
Koenig, JH;Ikeda, K
在本文中，证据表明单个终端内存在两种不同的突触小泡回收途径。一条途径从活性区发出，具有快速的时程，不涉及中间结构，并且通过暴露于高 Mg2+/低 Ca2+ 盐水而被阻断，而第二条途径从远离活性区的位点发出，具有较慢的时程，涉及内体中间体，并且对高 Mg2+/低 Ca2+ 不敏感。为了可视化这两种回收途径，使用了温度敏感的果蝇突变体 shibire，其中囊泡回收在 19°C 时正常，但在 29°C 时被阻断。当暴露于 29 摄氏度时，随着胞吐作用的进行而内吞作用被阻断，囊泡会完全耗尽。当温度降至26℃时，囊泡回收膜开始积聚，形成质膜内陷，但夹断被阻止。在这些实验条件下，可以通过电子显微镜分析区分这两个独立的途径。通过观察允许温度 19 摄氏度下的正常回收过程，进一步表征了这两种途径。这表明这两种回收途径的功能可能是产生两种不同的囊泡群体：活性区和非活性区群体。讨论了这两个种群具有不同释放特征和功能的可能性。
In this paper, evidence is presented that two distinct synaptic vesicle recycling pathways exist within a single terminal. One pathway emanates from the active zone, has a fast time course, involves no intermediate structures, and is blocked by exposure to high Mg2+/low Ca2+ saline, while the second pathway emanates at sites away from the active zone, has a slower time course, involves an endosomal intermediate, and is not sensitive to high Mg2+/low Ca2+. To visualize these two recycling pathways, the temperature-sensitive Drosophila mutant, shibire, in which vesicle recycling is normal at 19 degrees C but is blocked at 29 degrees C, was used. With exposure to 29 degrees C, complete vesicle depletion occurs as exocytosis proceeds while endocytosis is blocked. When the temperature is lowered to 26 degrees C, vesicle recycling membrane begins to accumulate as invaginations of the plasmalemma, but pinch-off is blocked. Under these experimental conditions, it was possible to distinguish the two separate pathways by electron microscopic analysis. These two pathways were further characterized by observing the normal recycling process at the permissive temperature, 19 degrees C. It is suggested that the function of these two recycling pathways might be to produce two distinct vesicle populations: the active zone and nonactive zone populations. The possibility that these two populations have different release characteristics and functions is discussed.
登录
查看更多内容
DOI：
10.1083/jcb.57.2.499
发表时间：
1973-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ceccarelli B;Hurlbut WP;Mauro A
通讯作者：
Mauro A
突变动力蛋白的诱导特异性阻断内吞涂层囊泡的形成。
影响因子：
7.8
作者：
Damke, H;Baba, T;Warnock, D E;Schmid, S L
通讯作者：
Schmid, S L
影响因子：
7.8
作者：
HEUSER, JE;REESE, TS
通讯作者：
REESE, TS
影响因子：
5.5
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS
影响因子：
56.9
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS