Increased oxidized protein hydrolase activity in serum and urine of diabetic rat models.
Increased oxidized protein hydrolase activity in serum and urine of diabetic rat models.
复制标题
糖尿病大鼠模型血清和尿液中氧化蛋白水解酶活性增加。
DOI：
10.1248/bpb.32.1632
复制
发表时间：
2009
影响因子：
2
通讯作者：
Tomoko Takahashi
中科院分区：
文献类型：
--
作者：
K. Shimizu;M. Ikegami;Tomoko Takahashi
氧化应激与糖尿病的发病机制有关。我们研究了氧化蛋白水解酶（OPH），它优先降解氧化和糖化蛋白，是否在糖尿病中具有预防或延迟作用。以链脲佐菌素（STZ）诱导的Wistar和Goto Kakizaki（GK）大鼠为1型和2型糖尿病模型，追踪血清和尿液中OPH活性随病理进展的变化。在两种大鼠模型中，血清OPH活性的增加几乎与血糖水平的增加平行，这在本研究中首先阐明了血清OPH活性从糖尿病的非常早期阶段开始增加。这些发现表明，血清OPH升高至少通过清除受损蛋白质对糖尿病的进展有延迟作用。尿OPH活性仅在STZ诱导的糖尿病大鼠伴微量白蛋白尿中升高，而在GK大鼠中无明显肾脏病变，提示循环OPH的渗漏;尿OPH活性升高可能是糖尿病肾病的一个有用的标志。OPH可能为糖尿病及其并发症提供一种新的治疗策略。
Oxidative stress is implicated in the pathogenesis of diabetes mellitus. We investigated whether or not oxidized protein hydrolase (OPH), which preferentially degrades oxidized and glycated proteins, has a preventive or delaying role in diabetes mellitus. Using streptozotocin (STZ)-induced diabetic Wistar and Goto-Kakizaki (GK) rats as models of types 1 and 2 diabetes, respectively, we traced the changes in serum and urinary OPH activity with the pathological progress. Serum OPH activity increased nearly in parallel with increases in the blood glucose level in the two rat models, clarifying first in this study that serum OPH activity increases from a very early stage of diabetes. These findings suggest that increased serum OPH has at least a delaying action against the progression of diabetes through the removal of damaged proteins. Urinary OPH activity increased only in STZ-induced diabetic rats with microalbuminuria, but not in GK rats still without marked renal disorder, indicating the leakage of circulating OPH; the increase in urinary OPH activity may be a useful marker for diabetic nephropathy. OPH may provide a new therapeutic strategy against diabetes and its complications.
DOI：
10.1093/ndt/11.supp5.13
发表时间：
1996
期刊：
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
影响因子：
--
作者：
O. Hori;S. Yan;S. Ogawa;K. Kuwabara;M. Matsumoto;D. Stern;A. Schmidt
通讯作者：
O. Hori;S. Yan;S. Ogawa;K. Kuwabara;M. Matsumoto;D. Stern;A. Schmidt
红细胞的酰肽水解酶活性。
DOI：
10.1016/0006-291x(85)90275-x
发表时间：
1985
影响因子：
3.1
作者：
Jones,WM;Manning,JM
通讯作者：
Manning,JM