EphrinB2 regulates osteogenic differentiation of periodontal ligament stem cells and alveolar bone defect regeneration in beagles
EphrinB2 regulates osteogenic differentiation of periodontal ligament stem cells and alveolar bone defect regeneration in beagles
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EphrinB2调节比格犬牙周膜干细胞的成骨分化和牙槽骨缺损再生
DOI：
10.1177/2041731419894361
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发表时间：
2019-01
影响因子：
8.2
通讯作者：
Yuan Changyong
中科院分区：
文献类型：
--
作者：
Wang Penglai;Wang Wen;Geng Tengyu;Liu Yi;Zhu Shaoyue;Liu Zongxiang;Yuan Changyong
EphrinB2是一种调节骨稳态的膜蛋白，已被证明可诱导牙周膜成纤维细胞中成骨基因的表达。本研究旨在探讨ephrinB2对牙周膜干细胞成骨分化及牙槽骨再生的影响。我们评估了ephrinB2修饰的人和犬牙周膜干细胞的成骨基因表达和成骨分化潜力，其中ephrinB2表达通过慢病毒载体转导上调。将EphrinB2修饰的犬牙周膜干细胞与PuraMatrix联合应用于比格犬临界大小的牙槽骨缺损，以评估骨再生。结果表明，ephrinB2过表达增强人和犬牙周膜干细胞的成骨基因转录和矿物质沉积。动物实验证实，与其他组相比，ephrinB2修饰的犬牙周膜干细胞+ PuraMatrix导致单位组织体积的骨小梁体积和骨小梁厚度更大。我们的研究表明，ephrinB2促进牙周膜干细胞的成骨分化和牙槽骨修复在比格犬，突出了其治疗牙槽骨损伤的治疗潜力。
EphrinB2, a membrane protein regulating bone homeostasis, has been demonstrated to induce osteogenic gene expression in periodontal ligament fibroblasts. The aim of this study was to explore the effects of ephrinB2 on osteogenic differentiation of periodontal ligament stem cells and on alveolar bone regeneration in vivo. We assessed the osteogenic gene expression and osteogenic differentiation potential of ephrinB2-modified human and canine periodontal ligament stem cells, in which ephrinB2 expression was upregulated via lentiviral vector transduction. EphrinB2-modified canine periodontal ligament stem cells combined with PuraMatrix were delivered to critical-sized alveolar bone defects in beagles to evaluate bone regeneration. Results showed that ephrinB2 overexpression enhanced osteogenic gene transcription and mineral deposition in both human and canine periodontal ligament stem cells. Animal experiments confirmed that ephrinB2-modified canine periodontal ligament stem cells + PuraMatrix resulted in greater trabecular bone volume per tissue volume and trabecular thickness compared with other groups. Our study demonstrated that ephrinB2 promoted osteogenic differentiation of periodontal ligament stem cells and alveolar bone repair in beagles, highlighting its therapeutic potential for the treatment of alveolar bone damage.
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压力调节成骨细胞和破骨细胞中的 ephrinB2 和 EphB4，有助于实验性牙齿移动过程中的牙槽骨吸收
影响因子：
1.9
作者：
Hou J;Chen Y;Meng X;Shi C;Li C;Chen Y;Sun H
通讯作者：
Sun H
影响因子：
3.9
作者：
Ortinau S;Schmich J;Block S;Liedmann A;Jonas L;Weiss DG;Helm CA;Rolfs A;Frech MJ
通讯作者：
Frech MJ
影响因子：
4.2
作者：
Dissanayaka, Waruna Lakmal;Zhu, Xiaofei;Jin, Lijian
通讯作者：
Jin, Lijian
影响因子：
29
作者：
Zhao, Chen;Irie, Naoko;Matsuo, Koichi
通讯作者：
Matsuo, Koichi
影响因子：
3
作者：
B. Heng;Shuai Wang;T. Gong;Jianguang Xu;C. Yuan;Chengfei Zhang
通讯作者：
B. Heng;Shuai Wang;T. Gong;Jianguang Xu;C. Yuan;Chengfei Zhang