Authentic Research Experience and "Big Data" Analysis in the Classroom: Maize Response to Abiotic Stress.
Authentic Research Experience and "Big Data" Analysis in the Classroom: Maize Response to Abiotic Stress.
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DOI：
10.1187/cbe.15-04-0081
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wiatros N
中科院分区：
文献类型：
--
作者：
Makarevitch I;Frechette C;Wiatros N
本文介绍了一个新的实验室系列的设计，实施和评估，旨在为学生提供真实的研究经验，在分析大型数据集，特别是在RNA-seq分析。将基于探究的方法融入课程正在改变本科课堂上科学的教学和学习方式。将定量推理和数学技能融入真实的生物学本科研究项目中，已被证明有利于学生培养未来科学家所需的各种技能，并吸引学生学习科学，技术，工程和数学学科。虽然大规模数据分析成为现代生物学研究的重要组成部分，但学生很少有机会参与大型生物数据集的分析。RNA-seq分析是一种允许精确测量基因组中所有基因的基因表达水平的工具，它彻底改变了分子生物学，并为学生参与真实研究提供了充足的机会。我们开发，实施和评估了一系列真实的研究实验室练习，将大数据RNA-seq分析纳入介绍性本科课堂。我们的实验室系列专注于分析玉米幼苗中响应非生物胁迫的基因表达变化;然而，它可以很容易地适应于任何其他生物系统的分析。对学生学习的客观和主观评估表明，他们在理解重要的生物学概念和与科学过程有关的技能方面取得了进展。
This article describes the design, implementation, and assessment of a novel laboratory series aimed at providing students with authentic research experience in analyzing large data sets, specifically in RNA-seq analysis. Integration of inquiry-based approaches into curriculum is transforming the way science is taught and studied in undergraduate classrooms. Incorporating quantitative reasoning and mathematical skills into authentic biology undergraduate research projects has been shown to benefit students in developing various skills necessary for future scientists and to attract students to science, technology, engineering, and mathematics disciplines. While large-scale data analysis became an essential part of modern biological research, students have few opportunities to engage in analysis of large biological data sets. RNA-seq analysis, a tool that allows precise measurement of the level of gene expression for all genes in a genome, revolutionized molecular biology and provides ample opportunities for engaging students in authentic research. We developed, implemented, and assessed a series of authentic research laboratory exercises incorporating a large data RNA-seq analysis into an introductory undergraduate classroom. Our laboratory series is focused on analyzing gene expression changes in response to abiotic stress in maize seedlings; however, it could be easily adapted to the analysis of any other biological system with available RNA-seq data. Objective and subjective assessment of student learning demonstrated gains in understanding important biological concepts and in skills related to the process of science.
DOI：
10.1187/cbe.10-03-0021
发表时间：
2010
期刊：
CBE life sciences education
影响因子：
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作者：
Milton JG;Radunskaya AE;Lee AH;de Pillis LG;Bartlett DF
通讯作者：
Bartlett DF