Extreme Sensitivity of Fitness to Environmental Conditions: Lessons from #1BigBatch.
Extreme Sensitivity of Fitness to Environmental Conditions: Lessons from #1BigBatch.
复制标题
DOI：
10.1007/s00239-023-10114-3
复制
发表时间：
2023-06
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
--
作者：
“适者生存”这句话已经成为自然选择如何运作的标志性描述。然而，精确测量适应性，即使是在受控的实验室条件下生长的单细胞微生物种群，仍然是一个挑战。虽然有许多方法可以进行这些测量，包括最近开发的利用DNA条形码的方法，但所有方法在区分适应度差异小的菌株的精度方面都受到限制。在这项研究中，我们排除了一些不精确的主要来源，但仍然发现适应性测量在重复之间存在很大差异。我们的数据表明，重复之间非常微妙且难以避免的环境差异会在适应度测量中产生系统性差异。最后，我们讨论了在极端环境依赖的情况下，适应度测量应该如何解释。这项工作的灵感来自于科学界，他们关注了我们，并在我们在#1BigBatch上直播了一个高重复的健身测量实验时给了我们建议。在线版本包含补充材料，可在10.1007/s00239-023-10114-3获得。
The phrase “survival of the fittest” has become an iconic descriptor of how natural selection works. And yet, precisely measuring fitness, even for single-celled microbial populations growing in controlled laboratory conditions, remains a challenge. While numerous methods exist to perform these measurements, including recently developed methods utilizing DNA barcodes, all methods are limited in their precision to differentiate strains with small fitness differences. In this study, we rule out some major sources of imprecision, but still find that fitness measurements vary substantially from replicate to replicate. Our data suggest that very subtle and difficult to avoid environmental differences between replicates create systematic variation across fitness measurements. We conclude by discussing how fitness measurements should be interpreted given their extreme environment dependence. This work was inspired by the scientific community who followed us and gave us tips as we live tweeted a high-replicate fitness measurement experiment at #1BigBatch. The online version contains supplementary material available at 10.1007/s00239-023-10114-3.
登录
查看更多内容
影响因子：
3.7
作者：
Geiler-Samerotte KA;Hashimoto T;Dion MF;Budnik BA;Airoldi EM;Drummond DA
通讯作者：
Drummond DA
影响因子：
3.9
作者：
Johnson, Milo S.;Venkataram, Sandeep;Kryazhimskiy, Sergey
通讯作者：
Kryazhimskiy, Sergey
影响因子：
48
作者：
Fowler, Douglas M.;Fields, Stanley
通讯作者：
Fields, Stanley
影响因子：
16.8
作者：
Jerison ER;Nguyen Ba AN;Desai MM;Kryazhimskiy S
通讯作者：
Kryazhimskiy S
影响因子：
3.3
作者：
Gresham, David;Boer, Viktor M.;Botstein, David
通讯作者：
Botstein, David