Characterization of the l-arabinofuranose-specific GafABCD ABC transporter essential for l-arabinose-dependent growth of the lignocellulose-degrading bacterium Shewanella sp. ANA-3.
Characterization of the l-arabinofuranose-specific GafABCD ABC transporter essential for l-arabinose-dependent growth of the lignocellulose-degrading bacterium Shewanella sp. ANA-3.
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L-阿拉伯呋喃糖特异性GAFABCD ABC转运蛋白的表征对于木质纤维素降解细菌SHEWANELLA SP的L-阿拉伯糖依赖性生长必不可少的表征。 ana-3。
DOI：
10.1099/mic.0.001308
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发表时间：
2023-03
期刊：
影响因子：
2.8
通讯作者：
Thomas, Gavin H
中科院分区：
文献类型：
--
作者：
Drousiotis, Konstantinos;Herman, Reyme;Hawkhead, Judith;Leech, Andrew;Wilkinson, Anthony;Thomas, Gavin H
在木质纤维素生物质上进化生存的微生物在有效和高效利用这种材料作为食物方面面临着独特的挑战。已知 Shewanella sp. ANA-3 细菌具有利用阿拉伯聚糖和阿拉伯木聚糖的潜力，而从这些聚合物中提取的单糖 l-arabinose 的吸收是由单一 ABC 转运体介导的。我们证明该系统的底物结合蛋白 GafASw 能与 l-arabinofuranose 特异性结合，而 l-arabinofuranose 是木质纤维素生物质中罕见的 l-arabinose 的呋喃糖形式。GafASw 的结构被解析到 1.7 Å，与大肠杆菌 YtfQ（GafAEc）的比较显示，其结合位点的适应性使其对呋喃糖而不是吡喃糖形式的单糖具有特异性，同时选择阿拉伯糖而不是另一种相关的单糖--半乳糖。发现一种细菌对某些木质纤维素材料中大量存在的一种糖具有天然的偏好，这表明在酶释放和吸收这种糖的过程中存在着密切的联系，也许是为了防止其他微生物在这种营养物质变异为 l-arabinopyranose 之前将其清除。这一生物学发现也为在工业生物技术中更有效地利用植物生物质成分提供了一条清晰的途径。
Microbes that have evolved to live on lignocellulosic biomass face unique challenges in the effective and efficient use of this material as food. The bacterium Shewanella sp. ANA-3 has the potential to utilize arabinan and arabinoxylan, and uptake of the monosaccharide, l-arabinose, derived from these polymers, is known to be mediated by a single ABC transporter. We demonstrate that the substrate binding protein of this system, GafASw, binds specifically to l-arabinofuranose, which is the rare furanose form of l-arabinose found in lignocellulosic biomass. The structure of GafASw was resolved to 1.7 Å and comparison to Escherichia coli YtfQ (GafAEc) revealed binding site adaptations that confer specificity for furanose over pyranose forms of monosaccharides, while selecting arabinose over another related monosaccharide, galactose. The discovery of a bacterium with a natural predilection for a sugar found abundantly in certain lignocellulosic materials suggests an intimate connection in the enzymatic release and uptake of the sugar, perhaps to prevent other microbes scavenging this nutrient before it mutarotates to l-arabinopyranose. This biological discovery also provides a clear route to engineer more efficient utilization of plant biomass components in industrial biotechnology.
影响因子：
2.9
作者：
Bagaria, Ashima;Kumaran, Desigan;Burley, Stephen K.;Swaminathan, Subramanyam
通讯作者：
Swaminathan, Subramanyam
影响因子：
3.7
作者：
Bourdès A;Rudder S;East AK;Poole PS
通讯作者：
Poole PS