Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.
Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.
复制标题
DOI：
10.1371/journal.pone.0110142
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Baba Y
中科院分区：
文献类型：
--
作者：
Yukawa H;Nakagawa S;Yoshizumi Y;Watanabe M;Saito H;Miyamoto Y;Noguchi H;Oishi K;Ono K;Sawada M;Kato I;Onoshima D;Obayashi M;Hayashi Y;Kaji N;Ishikawa T;Hayashi S;Baba Y
干细胞移植有望在再生医学中有各种应用。然而，为了检测和追踪体内移植的干细胞，需要非侵入性和广泛临床可用的细胞成像技术。本论文以磁共振成像技术为研究对象，探讨了本课题组新研制的三甲基氨基葡聚糖包裹的磁性氧化铁纳米颗粒-03（TMADM-03）作为对比剂标记脂肪组织来源的干细胞（ASCs）的可行性。在1小时转导时间后，在用小于100 µg-Fe/mL的TMADM-03转导的ASC中未观察到细胞毒性。TMADM-03到ASC中的转导效率比碱处理的葡聚糖包被的磁性氧化铁纳米颗粒（ATDM）的效率高约四倍，ATDM是市售造影剂如ferucarbotran（Resovist）的主要组分，并且标记水平维持至少两周。此外，证实了用TMADM-03标记的ASC的分化能力和它们产生细胞因子如肝细胞生长因子（HGF）、血管内皮生长因子（VEGF）和前列腺素E2（PGE 2）的能力得以维持。将TMADM-03标记的ASCs移植到小鼠的左肾包膜中。标记的ASCs可以使用1 T MR成像系统以良好的对比度成像。这些数据表明，TMADM-03因此可以用作干细胞MR成像的造影剂。
Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order to detect and trace the transplanted stem cells in the body, non-invasive and widely clinically available cell imaging technologies are required. In this paper, we focused on magnetic resonance (MR) imaging technology, and investigated whether the trimethylamino dextran-coated magnetic iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used for labeling adipose tissue-derived stem cells (ASCs) as a contrast agent. No cytotoxicity was observed in ASCs transduced with less than 100 µg-Fe/mL of TMADM-03 after a one hour transduction time. The transduction efficiency of TMADM-03 into ASCs was about four-fold more efficient than that of the alkali-treated dextran-coated magnetic iron oxide nanoparticle (ATDM), which is a major component of commercially available contrast agents such as ferucarbotran (Resovist), and the level of labeling was maintained for at least two weeks. In addition, the differentiation ability of ASCs labeled with TMADM-03 and their ability to produce cytokines such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), were confirmed to be maintained. The ASCs labeled with TMADM-03 were transplanted into the left kidney capsule of a mouse. The labeled ASCs could be imaged with good contrast using a 1T MR imaging system. These data suggest that TMADM-03 can therefore be utilized as a contrast agent for the MR imaging of stem cells.
登录
查看更多内容
影响因子：
4
作者：
Liu, Kaiyan;Chen, Yuhong;Huang, Xiaojun
通讯作者：
Huang, Xiaojun
影响因子：
4.8
作者：
Derossi, D;Calvet, S;Prochiantz, A
通讯作者：
Prochiantz, A
影响因子：
3.1
作者：
Mailaender, Volker;Lorenz, Myriam Ricarda;Schrezenmeier, Hubert
通讯作者：
Schrezenmeier, Hubert
影响因子：
6.4
作者：
Kazemi, Tohid;Asgarian-Omran, Hossein;Shokri, Fazel
通讯作者：
Shokri, Fazel
影响因子：
2.8
作者：
Arbab, Ali Syed;Yocum, Gene Thomus;Frank, Joseph Alan
通讯作者：
Frank, Joseph Alan