Appearance of differentiated cells derived from polar body nuclei in the silkworm, Bombyx mori.
Appearance of differentiated cells derived from polar body nuclei in the silkworm, Bombyx mori.
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DOI：
10.3389/fphys.2013.00235
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发表时间：
2013
影响因子：
4
通讯作者：
Suzuki MG
中科院分区：
文献类型：
--
作者：
Sakai H;Yokoyama T;Abe H;Fujii T;Suzuki MG
In Bombyx mori, polar body nuclei are observed until 9 h after egg lying, however, the fate of polar body nuclei remains unclear. To examine the fate of polar body nuclei, we employed a mutation of serosal cell pigmentation, pink-eyed white egg (pe). The heterozygous pe/+pe females produced black serosal cells in white eggs, while pe/pe females did not produce black serosal cells in white eggs. These results suggest that the appearance of black serosal cells in white eggs depends on the genotype (pe/+pe) of the mother. Because the polar body nuclei had +pe genes in the white eggs laid by a pe/+pe female, polar body nuclei participate in development and differentiate into functional cell (serosal cells). Analyses of serosal cells pigmentation indicated that ~30% of the eggs contained polar-body-nucleus-derived cells. These results demonstrate that polar-body-nucleus-derived cells appeared at a high frequency under natural conditions. Approximately 80% of polar-body-nucleus-derived cells appeared near the anterior pole and the dorsal side, which is opposite to where embryogenesis occurs. The number of cells derived from the polar body nuclei was very low. Approximately 26% of these eggs contained only one black serosal cell. PCR-based analysis revealed that the polar-body-nucleus-derived cells disappeared in late embryonic stages (stage 25). Overall, polar-body-nuclei-derived cells were unlikely to contribute to embryos.
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影响因子：
2.7
作者：
Tanaka, N;Yokoyama, T;Oshiki, T
通讯作者：
Oshiki, T
影响因子：
2.6
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Abe, H;Seki, M;Shimada, T
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Shimada, T
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2.7
作者：
Page, AW;OrrWeaver, TL
通讯作者：
OrrWeaver, TL
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4.6
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Pérez-Mongiovi, D;Malmanche, N;Sunkel, C
通讯作者：
Sunkel, C
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2.6
作者：
Yamamoto, Daisuke S.;Tachibana, Kazunori;Hatakeyama, Masatsugu
通讯作者：
Hatakeyama, Masatsugu