Compressive Buckling Fabrication of 3D Cell-Laden Microstructures.
Compressive Buckling Fabrication of 3D Cell-Laden Microstructures.
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DOI：
10.1002/advs.202101027
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Zhao R
中科院分区：
文献类型：
--
作者：
Chen Z;Anandakrishnan N;Xu Y;Zhao R
组织结构是其生物学功能的先决条件。重述三维(3D)组织结构是组织工程中最大的挑战之一。二维(2D)组织制造方法目前处于组织工程和疾病建模的主要阶段。然而，由于其平面性质，所创建的模型仅表示非常有限的平面外组织结构。在这里，利用压缩屈曲原理从微细加工的平面图案中创建3D仿生细胞负载微结构。这种方法允许以高空间精度进行细胞和细胞外基质图案的平面外传递。作为原理的证明，制作了包括盒子、章鱼、金字塔和连续波在内的各种聚合物3D微型结构。制作了一个具有空间分布的细胞陷窝结构的矿化骨组织模型，以验证该方法的制作能力。预计这种新的方法将有助于显著扩展已建立的用于3D组织制造的2D制造技术的用途。鉴于二维制造方法在生物医学研究中的广泛应用和对仿生三维结构的高要求，该方法有望弥合二维和三维组织制造之间的差距，并为组织工程和再生医学开辟新的可能性。这项工作是探索利用基于压缩屈曲的制造技术从原始的2D平面结构构建3D细胞加载微结构的可能性的第一次研究。预计这种新的方法将有助于显着扩大成熟的2D制造技术的用途，并在组织工程和再生医学中开辟新的可能性。
Tissue architecture is a prerequisite for its biological functions. Recapitulating the three‐dimensional (3D) tissue structure represents one of the biggest challenges in tissue engineering. Two‐dimensional (2D) tissue fabrication methods are currently in the main stage for tissue engineering and disease modeling. However, due to their planar nature, the created models only represent very limited out‐of‐plane tissue structure. Here compressive buckling principle is harnessed to create 3D biomimetic cell‐laden microstructures from microfabricated planar patterns. This method allows out‐of‐plane delivery of cells and extracellular matrix patterns with high spatial precision. As a proof of principle, a variety of polymeric 3D miniature structures including a box, an octopus, a pyramid, and continuous waves are fabricated. A mineralized bone tissue model with spatially distributed cell‐laden lacunae structures is fabricated to demonstrate the fabrication power of the method. It is expected that this novel approach will help to significantly expand the utility of the established 2D fabrication techniques for 3D tissue fabrication. Given the widespread of 2D fabrication methods in biomedical research and the high demand for biomimetic 3D structures, this method is expected to bridge the gap between 2D and 3D tissue fabrication and open up new possibilities in tissue engineering and regenerative medicine. This work represents the first study to explore the possibility of using compressive buckling‐based fabrication technology to build 3D cell‐laden microstructures from originally 2D planar structures. It is expected that this novel approach will help to significantly expand the utility of the well‐established 2D fabrication techniques and open up new possibilities in tissue engineering and regenerative medicine.
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影响因子：
--
作者：
Florencio-Silva R;Sasso GR;Sasso-Cerri E;Simões MJ;Cerri PS
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影响因子：
29.4
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DOI：
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期刊：
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DOI：
10.1126/science.1188302
发表时间：
2010-06-25
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
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影响因子：
6.8
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通讯作者：
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