Expression of beta 4 integrins in human skin: comparison of epidermal distribution with beta 1-integrin epitopes, and modulation by calcium and vitamin D3 in cultured keratinocytes.
Expression of beta 4 integrins in human skin: comparison of epidermal distribution with beta 1-integrin epitopes, and modulation by calcium and vitamin D3 in cultured keratinocytes.
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人类皮肤中 β 4 整合素的表达：表皮分布与 β 1 整合素表位的比较，以及培养的角质形成细胞中钙和维生素 D3 的调节。
DOI：
10.1111/1523-1747.ep12481896
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Uitto,J
中科院分区：
文献类型：
--
作者：
Ryynänen,J;Jaakkola,S;Engvall,E;Peltonen,J;Uitto,J
通过单克隆抗体免疫检测研究了成人和胎儿皮肤以及培养的角质形成细胞中β4整合素的表达，并与β1整合素的表达进行比较。 β1和β4整合素表位的分布在成人表皮中完全不同。正如我们之前报道的（J Clin Invest 84:19 16, 1989），β1 表位最显着地存在于外侧细胞-细胞接触点，而用 β4 抗体染色显示线性染色模式极化到与真皮表皮基底膜并置的基底表面。相比之下，在胎儿皮肤中，β1 和 β4 抗体的染色模式相似，表明基底和基底上角质形成细胞的整个细胞表面周围存在表位。在低钙 (0.15 mM) 培养基中培养的角质形成细胞中观察到 β4 整合素表位的明显极化，并且在人乳头瘤病毒转化的角质形成细胞中也注意到这些整合素的表达。将培养物转换为高钙 (1.2 mM) 培养基导致表位重新分布到某种模式，表明它们位于邻近基质的细胞下表面。这种 Ca++ 诱导的 β4 整联蛋白表位的重新分布可以被 10-7M 维生素 D3 抵消。最后，与抗β4整合素抗体一起孵育降低了角质形成细胞附着于塑料基质的能力。这些结果为β4整合素在细胞-基质相互作用中的作用提供了进一步的证据。
The expression of β4 integrins in adult and fetal human skin as well as in cultured keratinocytes was studied by immunodetection with monoclonal antibodies and compared with that of β1 integrins. The distribution of the β1 and β4 integrin epitopes was entirely different in the adult epidermis. As reported previously by us (J Clin Invest 84:19 16, 1989), the β1 epitopes were present most prominently at lateral cell-cell contact points, whereas staining with the β4 antibody demonstrated a linear staining pattern polarizing to the basal surface juxtaposed to the dermal-epidermal basement membrane. In contrast, in fetal skin, the staining patterns both with β1 and β4 antibodies were similar and demonstrated the presence of epitopes surrounding the entire cell surface of both basal and suprabasal keratinocytes. Distinct polarization of β4 integrin epitopes was noted in cultured keratinocytes maintained in low-calcium (0.15 mM) medium and the expression of these integrins was also noted in human papilloma virus-transformed keratinocytes. Switch of the cultures to high-calcium (1.2 mM) medium resulted in redistribution of the epitopes to a pattern suggesting their location at undersurface of the cells adjacent to the substratum. This Ca++- induced redistribution of β4 integrin epitopes could be counteracted by 10-7M vitamin D3. Finally, incubation with anti-β4 integrin antibody reduced the capacity of keratinocytes to attach to plastic substratum. The results provide further evidence for the role of β4 integrins in cell-matrix interactions.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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期刊：
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影响因子：
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