Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912.
Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912.
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短乳杆菌 NCL912 基于底物缓释的高效 GABA 生物合成
DOI：
10.1186/s12934-018-0919-6
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发表时间：
2018-05-19
影响因子：
6.4
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Wang Q;Liu X;Fu J;Wang S;Chen Y;Chang K;Li H
背景：γ-氨基丁酸(GABA)在食品和药品工业中发挥着重要作用。我们以前的研究建立了一种高效的补料分批发酵工艺，使简短乳杆菌NCL912利用L-谷氨酸单钠生产GABA，但L-谷氨酸单钠可能不是理想的底物，因为它会导致脱羧酸导致pH的快速上升。因此，本研究提出以L-谷氨酸为底物。结果：优化的初始发酵条件为：50g/L葡萄糖，25g/L酵母膏，10 mg/L硫酸锰(MnSO4.H2O)，2g/L吐温-80,220g/L L谷氨酸。葡萄糖、氮源、镁和吐温-80对L-谷氨酸工艺产生的GABA有显著影响，其他因素没有或几乎没有影响。优化后的发酵条件为：L葡萄糖25g/L、L酵母膏FM40825g/L、L MnSO4.H2O 25 mg/L、吐温-80 2g/L。短链乳杆菌NCL912在10 L发酵罐上进行了简单高效的GABA生物转化发酵工艺研究，确定的发酵条件为：发酵基质为5 L，谷氨酸为295g/L，接种量为10%(v/v)，培养温度为32℃，搅拌速度为100rpm。发酵48h后，最终的γ-氨基丁酸浓度可达205.8±8.0g/L。结论：L-谷氨酸比L-谷氨酸单钠更有利于γ-氨基丁酸的生物合成。从而建立了205g/L GABA对短链乳杆菌NCL912的高效生物发酵工艺。这一策略可能为提高GABA的生物转化率提供一种选择。
Background:Gamma-aminobutyric acid (GABA) plays a significant role in the food and drug industries. Our previous study established an efficient fed-batch fermentation process for Lactobacillus brevis NCL912 production of GABA from monosodium L-glutamate; however, monosodium L-glutamate may not be an ideal substrate, as it can result in the rapid increase of pH due to decarboxylation. Thus, in this study, L-glutamic acid was proposed as a substrate. To evaluate its potential, key components of the fermentation medium affecting GABA synthesis were re-screened and re-optimized to enhance GABA production from L. brevis NCL912.Results:The initial fermentation medium (pH 3.3) used for optimization was: 50 g/L glucose, 25 g/L yeast extract, 10 mg/L manganese sulfate (MnSO4·H2O), 2 g/L Tween-80, and 220 g/L L-glutamic acid. Glucose, a nitrogen source, magnesium, and Tween-80 had notable effects on GABA production from the L-glutamic acid-based process; other factors showed no or marginal effects. The optimized levels of the four key components in the fermentation medium were 25 g/L glucose, 25 g/L yeast extract FM408, 25 mg/L MnSO4·H2O, and 2 g/L Tween-80. A simple and efficient fermentation process for the bioconversion of GABA by L. brevis NCL912 was subsequently developed in a 10 L fermenter as follows: fermentation medium, 5 L; glutamic acid, 295 g/L; inoculum, 10% (v/v); incubation temperature, 32 °C; and agitation, 100 rpm. After 48 h of fermentation, the final GABA concentration increased up to 205.8 ± 8.0 g/L.Conclusions:L-Glutamic acid was superior to monosodium L-glutamate as a substrate in the bioproduction of GABA. Thus, a high efficacy bioprocess with 205 g/L GABA for L. brevis NCL912 was established. This strategy may provide an alternative for increasing the bioconversion of GABA.
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影响因子：
6.4
作者：
Li H;Qiu T;Huang G;Cao Y
通讯作者：
Cao Y
影响因子：
6.4
作者：
Kok J;Johansen E;Kleerebezem M;Teusink B
通讯作者：
Teusink B
影响因子：
4.4
作者：
Cotter, PD;Ryan, S;Hill, C
通讯作者：
Hill, C
影响因子：
3.8
作者：
Grewal, Jasneet;Khare, S. K.
通讯作者：
Khare, S. K.
影响因子：
3.6
作者：
Cotter, PD;Gahan, CGM;Hill, C
通讯作者：
Hill, C