Correlation of NADH and Ca2+ signals in mouse pancreatic acinar cells
Correlation of NADH and Ca2+ signals in mouse pancreatic acinar cells
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小鼠胰腺腺泡细胞中 NADH 和 Ca2 信号的相关性
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
A. Tepikin
中科院分区：
文献类型：
--
作者：
S. Voronina;T. Sukhomlin;Paul Johnson;G. Erdemli;O. Petersen;A. Tepikin
研究了钙释放分泌剂刺激的胰腺腺泡细胞钙信号和NADH反应之间的关系。用Fura Red或钙敏感Cl -电流研究胞质钙信号。用Rhod‐2测定线粒体钙含量。分泌颗粒区周围NADH自身荧光水平最高。用生理剂量的胆囊收缩素（CCK）刺激细胞触发NADH自身荧光的缓慢振荡。在分泌颗粒周围的线粒体团簇中NADH振荡被清楚地解决了。乙酰胆碱（ACh）诱导的极快的根尖钙信号对NADH无明显影响；稍微延长的根尖（或优先是根尖）钙瞬态触发了明显的NADH反应。胞质钙、线粒体钙和NADH的三重联合记录揭示了个体信号的发展顺序：胞质钙的增加伴随着线粒体钙反应的减慢，随后是NADH荧光的延迟增加。细胞质钙恢复快于线粒体钙恢复。线粒体钙水平升高时NADH恢复。在中剂量乙酰胆碱诱导的胞质钙瞬态振荡中，NADH荧光在第一次钙瞬态后出现初始增加；随后的每一种钙反应都产生双相NADH变化，包括最初的小幅下降，随后恢复到升高的钙水平。在高剂量乙酰胆碱诱导的高频率正弦钙振荡中，NADH反应融合成一个平滑的上升，然后缓慢下降。超大剂量乙酰胆碱和CCK产生单次大的NADH瞬态。
Relationships between calcium signals and NADH responses were investigated in pancreatic acinar cells stimulated with calcium‐releasing secretagogues. Cytosolic calcium signals were studied using Fura Red or calcium‐sensitive Cl− current. Mitochondrial calcium was measured using Rhod‐2. The highest levels of NADH autofluorescence were found around the secretory granule region. Stimulation of cells with physiological doses of cholecystokinin (CCK) triggered slow oscillations of NADH autofluorescence. NADH oscillations were clearly resolved in the mitochondrial clusters around secretory granules. Very fast apical calcium signals induced by acetylcholine (ACh) produced no detectable changes in NADH; slightly more extended apical (or preferentially apical) calcium transients triggered clear NADH responses. Triple combined recordings of cytosolic calcium, mitochondrial calcium and NADH revealed the sequence of development of individual signals: an increase in cytosolic calcium was accompanied by a slower mitochondrial calcium response followed by a delayed increase in NADH fluorescence. Recovery of cytosolic calcium was faster than recovery of mitochondrial calcium. NADH recovery occurred at elevated mitochondrial calcium levels. During the transient cytosolic calcium oscillations induced by intermediate doses of ACh, there was an initial increase in NADH fluorescence following the first calcium transient; each of the subsequent calcium responses produced biphasic NADH changes comprising an initial small decline followed by restoration to an elevated calcium level. During the higher‐frequency sinusoidal calcium oscillations induced by higher doses of ACh, NADH responses fused into a smooth rise followed by a slow decline. Supramaximal doses of ACh and CCK produced single large NADH transients.
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影响因子：
--
作者：
S. Arnaudeau;W. Kelley;J. Walsh;N. Demaurex
通讯作者：
S. Arnaudeau;W. Kelley;J. Walsh;N. Demaurex
DOI：
10.1085/jgp.116.4.547
发表时间：
2000-10
期刊：
The Journal of general physiology
影响因子：
--
作者：
Straub SV;Giovannucci DR;Yule DI
通讯作者：
Yule DI
影响因子：
4.3
作者：
Buntinas, L;Gunter, KK;Gunter, TE
通讯作者：
Gunter, TE
影响因子：
56.9
作者：
Rizzuto, R;Pinton, P;Pozzan, T
通讯作者：
Pozzan, T