Decrease of perforin positive CD3(+)γδ-T cells in patients with obstructive sleep disordered breathing.
Decrease of perforin positive CD3(+)γδ-T cells in patients with obstructive sleep disordered breathing.
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DOI：
10.1007/s11325-017-1602-6
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Moita LF
中科院分区：
文献类型：
--
作者：
Staats R;Rodrigues R;Barros A;Bacelar-Nicolau L;Aguiar M;Fernandes D;Moreira S;Simões A;Silva-Santos B;Rodrigues JV;Barbara C;de Almeida AB;Moita LF
睡眠相关呼吸障碍（SRBD）导致睡眠片段化、间歇性缺氧或两者的组合，从而导致包括免疫系统在内的稳态扰动。我们调查是否SRBD患者或无间歇性缺氧穿孔素和颗粒酶-B阳性外周血淋巴细胞表现出实质性差异。共纳入87名受试者，分布如下：24名对照组（C），19名因上气道阻力（UAR）增加而无缺氧事件的呼吸努力相关觉醒患者，24名患有阻塞性睡眠呼吸暂停（OSA）的肥胖患者（oOSA），20名无肥胖患者（noOSA）。在多导睡眠图记录后，我们分析了空腹血液样本的常规血液学和生化参数以及含有蛋白质穿孔素和颗粒酶B（GrB）的淋巴细胞的百分比。采用Kruskal-Wallis检验和后验多重比较对结果进行统计分析。穿孔蛋白阳性γδ-细胞显示组间存在显著差异（p = 0.017），尤其是对照组和oOSA组之间存在显著差异（p值= 0.04）;其余SRBD组也显示出与对照组的差异（C vs UAR：p = 0.08; C vs noOSA = 0.09），但未达到统计学显著性。SRBD组间无差异。颗粒酶B细胞在SRBD患者中减少，但差异无统计学意义。在其他研究的淋巴细胞亚群中未发现额外的统计学显著性结果。阻塞性睡眠呼吸障碍与穿孔素阳性CD 3 +γδ-T细胞减少相关虽然这一发现是在没有间歇性缺氧的瘦患者中检测到的，但只有在患有严重OSA的肥胖患者中才有统计学意义。由于CD 3 +γδ-T细胞在肿瘤细胞的控制中起着重要作用，我们的发现与OSA和癌症相关性的研究直接相关。本文的在线版本（10.1007/s11325-017-1602-6）包含补充材料，可供授权用户使用。
Sleep related breathing disorders (SRBD) cause sleep fragmentation, intermittent hypoxia or a combination of both leading to homeostasis perturbations, including in the immune system. We investigated whether SRBD patients with or without intermittent hypoxia show substantial differences in perforin and granzyme-B positive peripheral blood lymphocytes. A total of 87 subjects were included and distributed as follows: 24 controls (C), 19 patients with respiratory effort related arousals due to increased upper airway resistance (UAR) without hypoxic events, 24 obese patients with obstructive sleep apnea (OSA) (oOSA), and 20 without obesity (noOSA). After polysomnographic recording, we analyzed in fasting blood samples routine hematologic and biochemical parameters and the percentage of lymphocytes containing the proteins perforin and granzyme-B (GrB). Kruskal-Wallis tests and a posteriori multiple comparisons were applied for statistical analysis of results. Perforin-positive γδ-cells revealed significant differences between groups (p = 0.017), especially between the Control group and the oOSA (p-value = 0.04); the remaining SRBD groups also showed differences from the control (C vs UAR: p = 0.08; C vs noOSA = 0.09), but they did not raise to statistical significance. There were no differences among the SRBD groups. Granzyme-B cells were decreased in SRBD patients, but the differences were not statistically significant. No additional statistical significant result was found in the other investigated lymphocyte subsets. Obstructive sleep-disordered breathing is associated with a decrease in perforin-positive CD3+γδ-T cells. Although this finding was detected in lean patients without intermittent hypoxia, the reduction was only statistically significant in obese patients with severe OSA. Because CD3+γδ-T cells play an important role in the control of tumor cells, our findings are directly relevant for the study of the association of OSA and cancer. The online version of this article (10.1007/s11325-017-1602-6) contains supplementary material, which is available to authorized users.
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影响因子：
6.4
作者：
Addison, EG;North, J;Lowdell, MW
通讯作者：
Lowdell, MW
影响因子：
4.3
作者：
Nadeem, Rashid;Molnar, Janos;Loomba, Rohit
通讯作者：
Loomba, Rohit
影响因子：
7.8
作者：
Le Garff-Tavernier, Magali;Beziat, Vivien;Vieillard, Vincent
通讯作者：
Vieillard, Vincent
DOI：
10.1016/j.bbrc.2006.03.015
发表时间：
2006-05-05
影响因子：
3.1
作者：
Greenberg, H;Ye, XB;Liu, SF
通讯作者：
Liu, SF
影响因子：
37.8
作者：
Ryan, S;Taylor, CT;McNicholas, WT
通讯作者：
McNicholas, WT