Expression of the Myelin‐Associated Glycoprotein in Cultures of Immortalized Schwann Cells
Expression of the Myelin‐Associated Glycoprotein in Cultures of Immortalized Schwann Cells
复制标题
永生雪旺细胞培养物中髓磷脂相关糖蛋白的表达
DOI：
10.1111/j.1471-4159.1991.tb11432.x
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发表时间：
1991
影响因子：
4.7
通讯作者：
R. Quarles
中科院分区：
文献类型：
--
作者：
S. Goda;J. Hammer;D. Kobiler;R. Quarles
摘要：尽管在不存在神经元的情况下，在大鼠雪旺细胞的原代培养物中无法检测到髓鞘相关糖蛋白（MAG），但在一些通过重复传代而永生化的培养雪旺细胞系中证实了MAG表达。一种这样的许旺细胞系S-16的放射免疫测定显示出约1 ng/μg总蛋白的显著高的MAG浓度，该水平与成人坐骨神经中的MAG浓度相当。S-16细胞分裂非常迅速，比正常的许旺细胞更圆，并在达到高密度后精心制作许多过程。细胞半乳糖苷阳性，但表达很少或不表达P0糖蛋白或髓鞘碱性蛋白。与神经一样，培养细胞合成的MAG主要是较短的同种型（S-MAG）。此外，翻译后加工类似于体内发生的加工，包括相似程度的糖基化、寡糖硫酸化和多肽磷酸化。MAG对用胰蛋白酶或神经氨酸酶处理完整细胞的敏感性，以及对表面标记的敏感性，
Abstract: Although the myelin‐associated glycoprotein (MAG) cannot be detected in primary cultures of rat Schwann cells in the absence of neurons, MAG expression was demonstrated in some lines of cultured Schwann cells that had been immortalized by repetitive passaging. Radioimmunoassay of one such Schwann cell line, S‐16, showed a remarkably high MAG concentration of about 1 ng/μg of total protein, a level that is comparable to the MAG concentration in adult sciatic nerve. The S‐16 cells divide very rapidly, are rounder than normal Schwann cells, and elaborate many processes after reaching high density. The cells are galacto‐cerebroside positive, but express little or no P0 glycoprotein or myelin basic protein. As in nerve, the MAG synthesized by the cultured cells is primarily the shorter isoform (S‐MAG). Furthermore, the posttranslational processing resembles that occurring in vivo including a similar degree of glycosylation, sulfation of oligosaccharides, and phosphorylation of the polypeptide. The sensitivity of MAG to treatment of the intact cells with trypsin or neuraminidase, as well as surface labeling with ongroup
存在和不存在雪旺细胞轴突接触时髓磷脂相关糖蛋白基因的表达。
影响因子：
2.9
作者：
Gupta,SK;Poduslo,JF;Dunn,R;Roder,J;Mezei,C
通讯作者：
Mezei,C
影响因子：
56.9
作者：
SOBUE, G;PLEASURE, D
通讯作者：
PLEASURE, D
DOI：
10.1073/pnas.84.21.7768
发表时间：
1987-11
影响因子：
11.1
作者：
S. Porter;L. Glaser;R. Bunge
通讯作者：
S. Porter;L. Glaser;R. Bunge