Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
复制标题
蛛网膜酸和二十二碳六烯酸抑制人CD14+单核细胞中的破骨细胞形成和活性。
DOI：
10.1371/journal.pone.0125145
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Coetzee M
中科院分区：
文献类型：
--
作者：
Kasonga AE;Deepak V;Kruger MC;Coetzee M
不均衡的饮食会对健康产生不良影响。由于长链多不饱和脂肪酸（LCPUFA）需要包含在健康饮食中，因此一直是研究的焦点。然而，LCPUFAs对人破骨细胞形成和功能的影响尚未被探索。使用人CD 14+单核细胞分化模型来阐明ω-3 LCPUFA、二十二碳六烯酸（DHA）和ω-6 LCPUFA、花生四烯酸（AA）对破骨细胞形成和活性的影响。从健康供体的外周血中分离CD 14+单核细胞，并用巨噬细胞集落刺激因子和核因子κ B配体的受体激活剂刺激以产生破骨细胞。本研究的数据显示，当在分化的早期阶段处理时，两种LCPUFA均以剂量依赖性方式降低CD 14+单核细胞的破骨细胞形成潜力。此外，当暴露在破骨细胞分化的晚期阶段时，AA和DHA损害了成熟破骨细胞的骨吸收潜力，而不影响破骨细胞的数量。AA和DHA废除分化以及成熟破骨细胞中的玻连蛋白受体表达。相反，降钙素受体表达的抑制程度在LCPUFA之间变化，只有AA在破骨细胞分化过程中引起抑制。此外，AA和DHA下调破骨细胞分化和成熟破骨细胞中关键破骨细胞特异性基因的表达。本研究首次证明LCPUFAs可以调节人原代破骨细胞系中破骨细胞的形成和功能。
An unbalanced diet can have adverse effects on health. Long chain polyunsaturated fatty acids (LCPUFAs) have been the focus of research owing to their necessity of inclusion in a healthy diet. However, the effects of LCPUFAs on human osteoclast formation and function have not been explored before. A human CD14+ monocyte differentiation model was used to elucidate the effects of an ω-3 LCPUFA, docosahexaenoic acid (DHA), and an ω-6 LCPUFA, arachidonic acid (AA), on osteoclast formation and activity. CD14+ monocytes were isolated from peripheral blood of healthy donors and stimulated with macrophage colony stimulating factor and receptor activator of nuclear factor kappa-B ligand to generate osteoclasts. Data from this study revealed that both the LCPUFAs decreased osteoclast formation potential of CD14+ monocytes in a dose-dependent manner when treated at an early stage of differentiation. Moreover, when exposed at a late stage of osteoclast differentiation AA and DHA impaired the bone resorptive potential of mature osteoclasts without affecting osteoclast numbers. AA and DHA abrogated vitronectin receptor expression in differentiating as well as mature osteoclasts. In contrast, the degree of inhibition for calcitonin receptor expression varied between the LCPUFAs with only AA causing inhibition during osteoclast differentiation. Furthermore, AA and DHA down regulated the expression of key osteoclast-specific genes in differentiating as well as mature osteoclasts. This study demonstrates for the first time that LCPUFAs can modulate osteoclast formation and function in a human primary osteoclast cell line.
登录
查看更多内容
影响因子：
4.2
作者：
Farina, Emily K.;Kiel, Douglas P.;Tucker, Katherine L.
通讯作者：
Tucker, Katherine L.
影响因子：
7
作者：
Johnson, MR;Polymeropoulos, MH;Francomano, CA
通讯作者：
Francomano, CA
影响因子：
4.1
作者：
Ferlin, Alberto;Pepe, Anastasia;Foresta, Carlo
通讯作者：
Foresta, Carlo
DOI：
10.1016/b978-0-12-375670-1.10006-8
发表时间：
2011-01-01
期刊：
OSTEOIMMUNOLOGY: INTERACTIONS OF THE IMMUNE AND SKELETAL SYSTEMS
影响因子：
--
作者：
Faccio, Roberta;Choi, Yongwon;Takayanagi, Hiroshi
通讯作者：
Takayanagi, Hiroshi
影响因子：
3.7
作者：
Henriksen K;Flores C;Thomsen JS;Brüel AM;Thudium CS;Neutzsky-Wulff AV;Langenbach GE;Sims N;Askmyr M;Martin TJ;Everts V;Karsdal MA;Richter J
通讯作者：
Richter J