Preclinical Models of Neuroblastoma-Current Status and Perspectives.
Preclinical Models of Neuroblastoma-Current Status and Perspectives.
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作者：
神经母细胞瘤是一种起源于交感神经前体的儿科肿瘤。这种疾病以高度异质性而闻名。因此，发展足够的临床前模型来反映神经母细胞瘤的复杂生物学特性尤其具有挑战性。本文介绍了现有的神经母细胞瘤模型的现状及其优点和局限性，并展示了临床前神经母细胞瘤研究的未来前景。临床前、体外和体内模型仍然是癌症研究中不可或缺的工具。这些经典的模型，包括二维和三维细胞培养技术和动物模型，对于基础研究和翻译研究至关重要。然而，每个模型都有自己的局限性，通常不能完全概括人类疾病的过程。因此，迫切需要开发新的、先进的系统，以便能够有效地评估癌症发生和进展的机制，更准确地反映疾病的病理生理和复杂性，并有效地为患者的治疗决策提供信息。临床前模型对于神经母细胞瘤等罕见癌症尤其重要，在这些肿瘤中，可用于潜在治疗评估和筛查的患者来源样本的可用性有限。神经母细胞瘤的建模因疾病的异质性而进一步复杂化。在这篇综述中，我们介绍了神经母细胞瘤临床前模型的研究现状，讨论了它们的发展和特点，强调了优点和局限性，并描述了开发新的、更先进的和临床相关的方法的必要性。
Neuroblastoma is a pediatric tumor originating from the precursors of sympathetic nerves. The disease is known for its high heterogeneity. Hence, developing adequate preclinical models reflecting the complex biology of neuroblastoma is particularly challenging. This paper describes the current status of the available neuroblastoma models with their strengths and limitations, and demonstrates the future perspectives for preclinical neuroblastoma research. Preclinical in vitro and in vivo models remain indispensable tools in cancer research. These classic models, including two- and three-dimensional cell culture techniques and animal models, are crucial for basic and translational studies. However, each model has its own limitations and typically does not fully recapitulate the course of the human disease. Therefore, there is an urgent need for the development of novel, advanced systems that can allow for efficient evaluation of the mechanisms underlying cancer development and progression, more accurately reflect the disease pathophysiology and complexity, and effectively inform therapeutic decisions for patients. Preclinical models are especially important for rare cancers, such as neuroblastoma, where the availability of patient-derived specimens that could be used for potential therapy evaluation and screening is limited. Neuroblastoma modeling is further complicated by the disease heterogeneity. In this review, we present the current status of preclinical models for neuroblastoma research, discuss their development and characteristics emphasizing strengths and limitations, and describe the necessity of the development of novel, more advanced and clinically relevant approaches.
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DOI：
10.1126/science.aat6768
发表时间：
2018-12-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ackermann S;Cartolano M;Hero B;Welte A;Kahlert Y;Roderwieser A;Bartenhagen C;Walter E;Gecht J;Kerschke L;Volland R;Menon R;Heuckmann JM;Gartlgruber M;Hartlieb S;Henrich KO;Okonechnikov K;Altmüller J;Nürnberg P;Lefever S;de Wilde B;Sand F;Ikram F;Rosswog C;Fischer J;Theissen J;Hertwig F;Singhi AD;Simon T;Vogel W;Perner S;Krug B;Schmidt M;Rahmann S;Achter V;Lang U;Vokuhl C;Ortmann M;Büttner R;Eggert A;Speleman F;O'Sullivan RJ;Thomas RK;Berthold F;Vandesompele J;Schramm A;Westermann F;Schulte JH;Peifer M;Fischer M
通讯作者：
Fischer M
影响因子：
3.8
作者：
Kasemeier-Kulesa JC;Schnell S;Woolley T;Spengler JA;Morrison JA;McKinney MC;Pushel I;Wolfe LA;Kulesa PM
通讯作者：
Kulesa PM
影响因子：
4.6
作者：
Alothman, Sahar J.;Kang, Keunsoo;Liu, Xuefeng;Krawczyk, Ewa;Azhar, Redha, I;Hu, Rong;Goerlitz, David;Kallakury, Bhaskar, V;Furth, Priscilla A.
通讯作者：
Furth, Priscilla A.
DOI：
10.1073/pnas.69.1.258
发表时间：
1972-01-01
影响因子：
11.1
作者：
AMANO, T;NIRENBERG, M;RICHELSON, E
通讯作者：
RICHELSON, E
影响因子：
16.6
作者：
Ben Amar, Dounia;Thoinet, Karine;Villalard, Benjamin;Imbaud, Olivier;Costechareyre, Clelia;Jarrosson, Loraine;Reynaud, Florie;Novion Ducassou, Julia;Coute, Yohann;Brunet, Jean-Francois;Combaret, Valerie;Corradini, Nadege;Delloye-Bourgeois, Celine;Castellani, Valerie
通讯作者：
Castellani, Valerie