Sites of transcription of adenovirus type 5 genomes in relation to early viral DNA replication in infected HeLa cells. A high resolution in situ hybridization and autoradiographical study
Sites of transcription of adenovirus type 5 genomes in relation to early viral DNA replication in infected HeLa cells. A high resolution in situ hybridization and autoradiographical study
复制标题
5 型腺病毒基因组的转录位点与受感染 HeLa 细胞中早期病毒 DNA 复制相关。
DOI：
10.1016/0248-4900(91)90060-z
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
E. Puvion
中科院分区：
文献类型：
--
作者：
F. Puvion;E. Puvion
用原位杂交技术，在细胞核转化的中间阶段，观察了5型腺病毒（Ad5）感染HeLa细胞后病毒RNA分子的分布。通过短脉冲、高分辨率放射自显影术定位病毒DNA的转录位点。新生病毒RNA主要在外周复制区识别的核区室中发现，已知该区域是Ad 5病毒基因组的主要复制位点。病毒RNA分子也存在，但程度明显较低，在连续的单链（ss）DNA积累位点，另一个核内病毒诱导的结构，其中也发生了一些病毒基因组的复制。另外两个病毒诱导的核结构含有病毒RNA，偶尔异常增大的染色质间颗粒簇和致密环，这两种无DNA结构的意义未知，但可能在Ad5初级转录物的成熟过程中发挥作用。病毒信使RNA分子位于含有大量核糖体的细胞质的大面积上。我们分析了各种酶预处理的感染细胞的部分上的新生RNA的启示原位杂交的影响。
The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined byin situhybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription sites of the viral DNA were localized by short pulse, high resolution autoradiography. Nascent viral RNA was found mainly within the nuclear compartment identified at the peripheral replicative zone, which is known to be the main replicative site of Ad5 viral genomes. Viral RNA molecules also were present, but to a markedly lesser extent, within the contiguous single‐stranded (ss) DNA accumulation site, another intranuclear virus‐induced structure in which some replication of viral genomes also occurs. Two other virus‐induced nuclear structures contained viral RNA, the occasional exceptionally enlarged clusters of interchromatin granules and the compact rings, both DNA‐free structures of unknown significance but which might play a role in the process of maturation of the Ad5 primary transcripts. Viral messenger RNA molecules were localized over the large areas of the cytoplasm which contain numerous ribosomes. Our analysis of the effects of various enzymatic pretreatments of the sections of infected cells on the revelation of nascent RNA byin situhybridization is reviewed.
腺病毒 DNA 复制动力学 II。
影响因子：
3.7
作者：
Bodnar,JW;Pearson,GD
通讯作者：
Pearson,GD
腺病毒E1A的功能。
DOI：
--
发表时间：
1986
期刊：
Cancer surveys
影响因子：
--
作者：
Berk,AJ
通讯作者：
Berk,AJ