Bilayer lipid membranes from falling droplets
Bilayer lipid membranes from falling droplets
复制标题
落下的液滴形成的双层脂质膜
DOI：
10.1007/s00216-008-2588-5
复制
发表时间：
2009
影响因子：
4.3
通讯作者：
H. Morgan
中科院分区：
文献类型：
--
作者：
M. Zagnoni;M. Sandison;P. Marius;H. Morgan
摘要我们描述了一种系统，它提供了一种快速而简单的方法，在微流体平台内从水性液滴形成悬浮的脂质双层。通过接触在两相液-液界面处形成的单层，在聚合物装置中产生双层脂质膜。含有膜蛋白的微滴被注入到位于孔上方的电极上，该孔通过填充有脂质烷烃溶液的圆锥形腔体加工而成。BLM的形成仅取决于装置的几何形状，并且仅通过分配缓冲液滴而导致脂质双层的自发形成。当注射含有跨膜蛋白或蛋白脂体的水滴时，可以进行直接的电生理测量。该方法适用于并入芯片实验室设备，并允许缓冲液交换和电测量。
图双层脂质膜是在聚合物装置中通过将含有膜蛋白的水滴直接注射到位于孔上方的电极上形成的，该孔被加工成锥形腔，该腔最初填充有脂质-烷烃溶液。水滴沿着电极滑到圆锥形容器底部的孔。该系统的几何形状使得能够自发形成BLM。在底部通道中的电极和液滴中的电极之间记录离子通道活性。该技术是可扩展的，并且可以被配置为高通量多位点生物传感或药物筛选平台。
AbstractWe describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid–liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid–alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements.
FigureBilayer lipid membranes are formed in a polymeric device by injecting water droplets, containing membrane proteins, directly onto an electrode positioned above an aperture machined into a conical cavity, which is initially filled with a lipid-alkane solution. The water droplet slides down the electrode to the aperture at the bottom of the conical reservoir. The geometry of this system enables the spontaneous formation of a BLM. Ion channel activity is recorded between an electrode in the bottom channel and the electrode in the droplet. The technique is scalable and could be configured as a high throughput multi-site biosensing or drug screening platform.
影响因子：
15
作者：
Holden, Matthew A.;Needham, David;Bayley, Hagan
通讯作者：
Bayley, Hagan