Urinary microRNA biomarkers for detecting the presence of esophageal cancer.
Urinary microRNA biomarkers for detecting the presence of esophageal cancer.
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DOI：
10.1038/s41598-021-87925-1
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发表时间：
2021-04-20
影响因子：
4.6
通讯作者：
Kataoka H
中科院分区：
文献类型：
--
作者：
Okuda Y;Shimura T;Iwasaki H;Fukusada S;Nishigaki R;Kitagawa M;Katano T;Okamoto Y;Yamada T;Horike SI;Kataoka H
食管癌（EC），包括食管鳞状细胞癌（ESCC）和腺癌（EAC）通常表现出不良预后;因此，能够早期检测的非侵入性生物标志物是必要的。将年龄和性别匹配的150名健康对照（HC）和43名ESCC患者随机分为两组：9名个体在发现队列中进行微阵列分析，184名个体在训练/测试队列中进行交叉验证进行qRT-PCR分析。使用152个尿液样本（144个HC和8个EAC），我们验证了用于EAC诊断的尿miRNA生物标志物。在发现队列中选择的八种miRNA中，在训练/测试队列中，ESCC组中五种miRNA（miR-1273 f、miR-619- 5 p、miR-150- 3 p、miR-4327和miR-3135 b）的尿液水平显著高于HC组。一致地，这五种尿miRNAs在训练集和测试集中在HC和ESCC之间显著不同。尤其是，尿miR-1273 f和miR-619- 5 p显示出用于诊断I期ESCC的曲线下面积（AUC）≥ 0.80的优异值。类似地，EAC组的这五种miRNA的尿水平显著高于HC组，AUC值约为0.80。本研究建立了新的尿miRNA生物标志物，可以早期检测ESCC和EAC。
Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randomly divided into two groups: 9 individuals in the discovery cohort for microarray analysis and 184 individuals in the training/test cohort with cross-validation for qRT-PCR analysis. Using 152 urine samples (144 HCs and 8 EACs), we validated the urinary miRNA biomarkers for EAC diagnosis. Among eight miRNAs selected in the discovery cohort, urinary levels of five miRNAs (miR-1273f, miR-619-5p, miR-150-3p, miR-4327, and miR-3135b) were significantly higher in the ESCC group than in the HC group, in the training/test cohort. Consistently, these five urinary miRNAs were significantly different between HC and ESCC in both training and test sets. Especially, urinary miR-1273f and miR-619-5p showed excellent values of area under the curve (AUC) ≥ 0.80 for diagnosing stage I ESCC. Similarly, the EAC group had significantly higher urinary levels of these five miRNAs than the HC group, with AUC values of approximately 0.80. The present study established novel urinary miRNA biomarkers that can early detect ESCC and EAC.
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