Comparative study of Arabidopsis PBS1 and a wheat PBS1 homolog helps understand the mechanism of PBS1 functioning in innate immunity.
Comparative study of Arabidopsis PBS1 and a wheat PBS1 homolog helps understand the mechanism of PBS1 functioning in innate immunity.
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DOI：
10.1038/s41598-017-05904-x
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发表时间：
2017-07-14
影响因子：
4.6
通讯作者：
Lu D
中科院分区：
文献类型：
--
作者：
Sun J;Huang G;Fan F;Wang S;Zhang Y;Han Y;Zou Y;Lu D
拟南芥 AVRPPHB SUSCEPTIBLE1 (PBS1) 在被丁香假单胞菌效应子 Pseudomonas Phaseolicola B (AvrPphB) 裂解后，充当“诱饵”激活对丁香假单胞菌 5 (RPS5) 的抵抗。 PBS1 中的 SEMPH 基序被认为可以通过 RPS5 将其与密切相关的拟南芥激酶区分开来。然而，其根本机制尚不完全清楚。在这里，我们分离并表征了小麦 PBS1 同源物 TaPBS1。尽管这种质膜定位激酶可以被 AvrPphB 裂解并且可以与 RPS5 结合，但它在瞬时测定中未能触发 RPS5 介导的超敏反应 (HR)。 TaPBS1 含有 STRPH 基序。 RPS5 与 TaPBS1 的关联弱于与 PBS1 的关联。将 STRPH 基序更改为 SEMPH 基序使得 TaPBS1 能够触发 HR。然而，PBS1 与 RPS5 的关联不需要 SEMPH 基序。 “SEMPH”和“STRPH”之间的差异表明了“EM”在 PBS1 中的重要性。此外，我们发现 SEMPH 基序中“E”位置处的带负电荷的氨基酸是 RPS5 识别 PBS1 所必需的。此外，PBS1 和 TaPBS1 都会经历鞭毛蛋白诱导的磷酸化。因此，我们的工作将有助于了解 PBS1 在植物先天免疫中发挥作用的机制。
Arabidopsis AVRPPHB SUSCEPTIBLE1 (PBS1) serves as a “decoy” in activating RESISTANCE TO PSEUDOMONAS SYRINGAE5 (RPS5) upon cleavage by Pseudomonas phaseolicola B (AvrPphB), a Pseudomonas syringae effector. The SEMPH motif in PBS1 was thought to allow it to be distinguished by RPS5 from the closely related Arabidopsis kinases. However, the underlying mechanism is not fully understood. Here, we isolated and characterized a wheat PBS1 homolog, TaPBS1. Although this plasma membrane-localized kinase could be cleaved by AvrPphB and could associate with RPS5, it failed to trigger RPS5-mediated hypersensitive response (HR) in a transient assay. TaPBS1 harbors a STRPH motif. The association of RPS5 with TaPBS1 was weaker than with PBS1. Change of the STRPH motif to the SEMPH motif allowed TaPBS1 to trigger HR. However, the SEMPH motif is not required for association of PBS1 with RPS5. The difference between “SEMPH” and “STRPH” points to the importance of “EM” in PBS1. Furthermore we found that a negatively charged amino acid at the position of “E” in the SEMPH motif was required for recognition of PBS1 by RPS5. Additionally, both PBS1 and TaPBS1 undergo the flagellin-induced phosphorylation. Therefore, our work will help understand the mechanism of PBS1 functioning in plant innate immunity.
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影响因子：
64.8
作者：
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DOI：
10.1073/pnas.0909705107
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