Wheat Gene TaATG8j Contributes to Stripe Rust Resistance.
Wheat Gene TaATG8j Contributes to Stripe Rust Resistance.
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小麦基因 TaATG8j 有助于抗条锈病
DOI：
10.3390/ijms19061666
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发表时间：
2018-06-05
影响因子：
5.6
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Mamun MA;Tang C;Sun Y;Islam MN;Liu P;Wang X;Kang Z
据报道，自噬相关蛋白8 （Autophagy-related 8, ATG8）参与了植物的先天免疫反应，但目前尚不清楚这些基因是否在谷类作物对抗专性生物营养真菌病原体中发挥类似作用。本文报道了一种来自小麦（Triticum aestivum）的ATG8基因，命名为TaATG8j。该基因有三个拷贝，分别位于2AS、2BS和2DS染色体上。小麦品种水原11号接种了条锈病病原菌小麦锈病锈菌（Pst）无毒小种CYR23后，这3个拷贝的转录量均上调。TaATG8j在烟叶中的瞬时表达表明，TaATG8j蛋白分布于整个细胞质中，但主要分布在细胞核和质膜中。TaATG8j在benthamiana中的过表达略微延迟了小鼠凋亡蛋白BAX （bcl2相关X蛋白）引起的细胞死亡。然而，TaATG8j在酵母（Schizosaccharomyces pombe）中的表达可诱导细胞死亡。病毒诱导的所有TaATG8j拷贝的基因沉默使水原11号对无毒的Pst小种CYR23敏感，同时真菌生物量增加，每个感染部位的坏死面积减少。上述结果表明，TaATG8j基因通过调控细胞死亡参与小麦对条锈病的抗性，为进一步了解小麦对条锈病的抗性机制提供了信息。
Autophagy-related 8 (ATG8) protein has been reported to be involved in plant’s innate immune response, but it is not clear whether such genes play a similar role in cereal crops against obligate biotrophic fungal pathogens. Here, we reported an ATG8 gene from wheat (Triticum aestivum), designated TaATG8j. This gene has three copies located in chromosomes 2AS, 2BS, and 2DS. The transcriptions of all three copies were upregulated in plants of the wheat cultivar Suwon 11, inoculated with an avirulent race (CYR23) of Puccinia striiformis f. sp. tritici (Pst), the causal fungal pathogen of stripe rust. The transient expression of TaATG8j in Nicotiana benthamiana showed that TaATG8j proteins were distributed throughout the cytoplasm, but mainly in the nucleus and plasma membrane. The overexpression of TaATG8j in N. benthamiana slightly delayed the cell death caused by the mouse apoptotic protein BAX (BCL2-associated X protein). However, the expression of TaATG8j in yeast (Schizosaccharomyces pombe) induced cell death. The virus-induced gene silencing of all TaATG8j copies rendered Suwon 11 susceptible to the avirulent Pst race CYR23, accompanied by an increased fungal biomass and a decreased necrotic area per infection site. These results indicate that TaATG8j contributes to wheat resistance against stripe rust fungus by regulating cell death, providing information for the understanding of the mechanisms of wheat resistance to the stripe rust pathogen.
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DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
64.5
作者：
Hofius, Daniel;Schultz-Larsen, Torsten;Petersen, Morten
通讯作者：
Petersen, Morten
小麦过敏诱导反应基因TaHIR1和TaHIR3参与对条锈菌感染和非生物胁迫的反应
影响因子：
6.2
作者：
Duan, Yinghui;Guo, Jun;Kang, Zhensheng
通讯作者：
Kang, Zhensheng
影响因子：
64.5
作者：
Liu, Y;Schiff, M;Dinesh-Kumar, SP
通讯作者：
Dinesh-Kumar, SP
影响因子：
7.2
作者：
Lenz, Heike D.;Haller, Eva;Nuernberger, Thorsten
通讯作者：
Nuernberger, Thorsten