gofasta: command-line utilities for genomic epidemiology research.

gofasta: command-line utilities for genomic epidemiology research.
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DOI:
10.1093/bioinformatics/btac424
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发表时间:
2022-08-10
期刊:
影响因子:
5.8
通讯作者:
Jackson, Ben
Jackson, Ben
中科院分区:
生物学3区
文献类型:
--
作者:
Jackson, Ben

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gofasta包括一组命令行实用程序,用于在基因组流行病学背景下处理短组装基因组的比对。它是为处理大量密切相关的SARS-CoV-2病毒基因组而开发的,应该对其他密集采样的病原体基因组数据集有用。它提供的功能是将组装的基因组之间的sam格式成对比对转换为fasta格式;注释多个序列比对中的突变,并通过遗传距离测量提取序列集用于疫情调查。gofasta是一个在MIT许可下发布的开源项目。二进制文件可以从Bioconda的https://github.com/virus-evolution/gofasta获得,也可以通过Go编程语言的包管理系统获得。源代码和进一步的文档,包括常见用例的walktool,可在GitHub存储库中获得。
gofasta comprises a set of command-line utilities for handling alignments of short assembled genomes in a genomic epidemiology context. It was developed for processing large numbers of closely related SARS-CoV-2 viral genomes and should be useful with other densely sampled pathogen genomic datasets. It provides functions to convert sam-format pairwise alignments between assembled genomes to fasta format; to annotate mutations in multiple sequence alignments, and to extract sets of sequences by genetic distance measures for use in outbreak investigations. gofasta is an open-source project distributed under the MIT license. Binaries are available at https://github.com/virus-evolution/gofasta, from Bioconda, and through the Go programming language’s package management system. Source code and further documentation, including walkthroughs for common use cases, are available on the GitHub repository.
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