APPLICATION OF MRNA IN SITU HYBRIDIZATION TO SURGICAL PATHOLOGY MATERIALS
APPLICATION OF MRNA IN SITU HYBRIDIZATION TO SURGICAL PATHOLOGY MATERIALS
复制标题
mRNA原位杂交在手术病理材料中的应用
DOI：
10.1267/ahc.27.567
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发表时间：
1994
影响因子：
2.4
通讯作者：
H. Sasano
中科院分区：
文献类型：
--
作者：
H. Sasano
mRNA原位杂交在生物学和医学的各个领域提供了重要的信息，但与免疫组织化学相比，将该技术纳入诊断病理学实验室并不容易。该技术是相对繁琐和耗时的，它是重要的病理学家，以确定在什么情况下mRNA原位杂交可以提供重要的信息，在外科病理材料。现将利用手术病理材料进行mRNA原位杂交的生物学意义总结如下：i）确定通过常规免疫组织化学观察到的免疫反应性是否代表产生的、储存的或与受体结合的基因产物，ii）研究具有快速细胞内半衰期的基因产物的定位，iii）当DNA序列已知但没有可靠的抗体可用于免疫组织化学时，检查表达的定位。和iv）如果mRNA和蛋白质水平的表达之间存在差异，则分析这两个水平的表达。外科病理学家在进行和/或订购mRNA原位杂交之前应考虑上述这些方面。无论采用何种固定剂，快速固定对于标本制备也很重要。探针和检测方法的选择可能会影响结果，但重要的是病理学家根据设施和预算在自己的实验室中确定最合适的方法。在一般情况下，mRNA在组织标本中的保存和检测系统的灵敏度在很大程度上影响mRNA原位杂交对外科病理材料的结果。
mRNA in situ hybridization has provided important information in the various fields of biology and medicine but it is not easy to incorporate the technique to diagnostic pathology laboratories, compared to immunohistochemistry. The technique is relatively cumbersome and time-consuming and it is important for pathologists to determine in what situations mRNA in situ hybridization can provide important information in surgical pathology materials. Biological significance of mRNA in situ hybridization employing surgical pathology materials are summarized as follows; i) determine whether immunoreactivity observed by conventional immunohistochemistry represent the gene products produced, stored or bound to receptor, ii) study the localization of the gene products with rapid intracellular half-life, iii) examine the localization of expression when DNA sequences are known but no reliable antibodies are available for immunohistochemistry. and iv) analyze the expression at mRNA and protein levels if there are discrepancies between these two levels of expression. Surgical pathologists should consider these aspects above prior to performing and/or ordering mRNA in situ hybridization. Prompt fixation is also important for specimen preparation regardless of fixatives employed. Choice of the probes and detection methods may influence the results but it is important for pathologists to determine the most appropriate methods in his or her own laboratories based on the facility and budget. In general, the preservation of mRNA in tissue specimen and sensitivity of detection systems largely influence the results of mRNA in situ hybridization on surgical pathology materials.
影响因子：
5.8
作者：
INKSTER, SE;BRODIE, AMH
通讯作者：
BRODIE, AMH
人类卵巢中芳香酶、17 α-羟化酶和侧链裂解细胞色素 P-450 的免疫定位。
DOI：
10.1530/jrf.0.0850163
发表时间：
1989
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Sasano,H;Okamoto,M;Mason,JI;Simpson,ER;Mendelson,CR;Sasano,N;Silverberg,SG
通讯作者：
Silverberg,SG